토끼풀에서 분리한 Peanut stunt virus의 성질 = Some Properties of an Isolate of Peanut stunt virus Isolated from White Clover (Trifolium repens L.)

다국어 초록 (Multilingual Abstract)

An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L)
showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced
local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic
symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control
virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of
four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by
RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of
approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological
properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first
report on the occurrence of PSV in white clover in Korea.

다국어 초록 (Multilingual Abstract)

An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L)
showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced
local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic
symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control
virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of
four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by
RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of
approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological
properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first
report on the occurrence of PSV in white clover in Korea.

참고문헌 (Reference)

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