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      2-Bromoacetylnaphthalene을 螢光誘導體化劑로 利用한 Alclofenac의 HPLC 分析에 관한 硏究 = Study on the HPLC Determination of Alclofenac Using 2-Bromoacetylnaphthalene as a Derivatizing Reagent

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      https://www.riss.kr/link?id=A19595428

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      A simple, rapid and high sensitive method for determination of alclofenac is described. 2-Bromoacetylnaphthalene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. Alclofenac was derivatized quantitatively into fluorescent compound by reacting with 2-bromoacetylnaphthalene in the presence of 18-crown-6 ether in acetonitrile.
      The optimum conditions for the derivatization such as concentration of KOH, 18-crown-6 and 2-bromoacetylnaphthalene, reaction temperature and reaction time were investigated.
      The structure of alcolofenac derivative was confirmed from IR, NMR and mass spectra. The fluorescence properties of alclofenac derivative were examined.
      The derivative was separated on a reverse phase column (Lichrosorb RP-8) in isocratid elution mode using the secondary mixture of acetonitrile and water as the mobile phase. The effluent was monitored by fluorometer.(Ex. 303, Em. 418 nm).
      The calibration plots for the peak area versus concentration of alclofenac observed to be linear(r =0.999).
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      A simple, rapid and high sensitive method for determination of alclofenac is described. 2-Bromoacetylnaphthalene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. Alclofenac was derivatized quantit...

      A simple, rapid and high sensitive method for determination of alclofenac is described. 2-Bromoacetylnaphthalene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. Alclofenac was derivatized quantitatively into fluorescent compound by reacting with 2-bromoacetylnaphthalene in the presence of 18-crown-6 ether in acetonitrile.
      The optimum conditions for the derivatization such as concentration of KOH, 18-crown-6 and 2-bromoacetylnaphthalene, reaction temperature and reaction time were investigated.
      The structure of alcolofenac derivative was confirmed from IR, NMR and mass spectra. The fluorescence properties of alclofenac derivative were examined.
      The derivative was separated on a reverse phase column (Lichrosorb RP-8) in isocratid elution mode using the secondary mixture of acetonitrile and water as the mobile phase. The effluent was monitored by fluorometer.(Ex. 303, Em. 418 nm).
      The calibration plots for the peak area versus concentration of alclofenac observed to be linear(r =0.999).

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