가토 두개골 결손부에서 합성골이식재와 혼합사용된 혈소판풍부혈장 (PRP)과 혈소판풍부피브린(PRF) 적용에 따른 골형성 효과비교 = Comparative Study on the Bone Formation Effect of Platelet Rich Plasma(PRP) and Platelet Rich Fibrin(PRF) in Combination with the Synthetic Bone Materials in Rabbit Cranial Defects

다국어 초록 (Multilingual Abstract)

To evaluate the clinical applicability of using PRF mixed with the synthetic bone materials compared to using PRP and no surgical additives. Four bicortical skull defects were prepared in 4 New Zealand white rabbits. In each, 1 defect was used as control and 3 defects were filled with HA/β-TCP, PRP mixed with HA/β-TCP, and PRF mixed with HA/β-TCP separately. After 8weeks, the rabbits were sacrificed and new bone formation was assessed by soft x-ray, conebeam CT and the histomorphometric analysis. Compared tocontrol group, the others showed remarkable bone formations. And PRP and PRF groups showed better results than no additive group. But there was no significant difference between PRP and PRF. The findings demonsatrates that the addition of PRP or PRF enhances bone formation. Though we cannot conclude PRF is superior to PRP from this study only, PRF can help the bone healing.

참고문헌 (Reference)

1 최정원, "치조골 재생용 인공재료의 요구조건" 한국조직공학과 재생의학회 4 (4): 478-483, 2007

2 윤소희, "젤라틴을 함유한 생분해성 나노섬유의 제조 및 특성" 한국조직공학과 재생의학회 5 (5): 835-841, 2008

3 전성현, "뼈이식재의 최근 연구동향" 한국조직공학과 재생의학회 5 (5): 791-797, 2008

4 G Sammartino, "Use of autologous platelet-rich plasma(PRP) in periodontal defect treatment after extraction of impacted mandibular third molars" 63 : 766-, 2005

5 AS Plachokova, "The bone regenerative effect of platelet-rich plasma in combination with an osteoconductive material in rat cranial defects" 17 : 305-, 2006

6 EG Freymiller, "Platelet-rich plasma: ready or not" 62 : 484-, 2004

7 RE Marx, "Platelet-rich plasma: evidence to support its use" 62 : 489-, 2004

8 RE Marx, "Platelet-rich plasma: Growth factor enhancement for bone grafts" 85 : 638-, 1998

9 RE Marx, "Platelet-rich plasma(PRP): what is PRP and what is not PRP" 10 : 225-, 2001

10 VS Raja, "Platelet-rich fibrin: evolution of a secondgeneration platelet concentrate" 19 : 42-, 2008

11 J Choukroun, "Platelet-rich fibrin(PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift" 101 : 299-, 2006

12 J Choukroun, "Platelet-rich fibrin(PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing" 101 : 56-, 2006

13 DM Dohan, "Platelet-rich fibrin(PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates" 101 : 51-, 2006

14 DM Dohan, "Platelet-rich fibrin(PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features" 101 : 45-, 2006

15 DM Dohan, "Platelet-rich fibrin(PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution" 101 : 37-, 2006

16 E Anitua, "Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants" 14 : 529-, 1999

17 A Diss, "Osteotome sinus floor elevation using Choukroun's platelet-rich fibrin as grafting material: a 1-year prospective pilot study with microthreaded implants" 105 : 572-, 2008

18 TB Jensen, "No effect of plateletrich plasma with frozen or processed bone allograft around noncemented implants" 29 : 67-, 2005

19 C Meyer, "Massive sinus-lift procedures with beta-tricalcium phosphate: Long-term results" 2009

20 AR Sanchez, "Is platelet-rich plasma the perfect enhancement factor? A current review" 18 : 93-, 2003

21 TL Aghaloo, "Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study" 60 : 1176-, 2002

22 R Ewers, "Histologic findings at augmented bone areas supplied with two different bone substitute materials combined with sinus floor lifting. Report of one case" 15 : 96-, 2004

23 Z Suba, "Facilitation of betatricalcium phosphate-induced alveolar bone regeneration by platelet-rich plasma in beagle dogs: a histologic and histomorphometric study" 19 : 832-, 2004

24 AE Turk, "Enhanced healing of large cranial defects by an osteoinductive protein in rabbits" 92 : 593-, 1993

25 김선종, "Effects of rhBMP-2 Coating Tricalcium Phosphate on Socket Preservation in Dog Extraction Socket" 한국조직공학과 재생의학회 5 (5): 637-642, 2008

26 AS Plachokova, "Early effect of platelet-rich plasma on bone healing in combination with an osteoconductive material in rat cranial defects" 18 : 244-, 2007

27 G Daculsi, "Current state of the art of biphasic calcium phosphate bioceramics" 14 : 195-, 2003

28 SS Jensen, "Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate: A histologic and histomorphometric study in the mandibles of minipigs" 17 : 237-, 2006