Brazilin Inhibits of TPA-induced MMP-9 Expression Via the Suppression of NF-κB Activation in MCF-7 Human Breast Carcinoma Cells

다국어 초록 (Multilingual Abstract)

Metastasis is the primary cause of from breast cancer mortality. Cell migration and invasion play important roles in neoplastic metastasis. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. NF-κB is transcription factor important in the regulation of MMP-9, as the promoter of MMP-9 gene contains binding sites for NF-κB. Brazilin, an active component of sappan wood (Caesalpinia sappan), decreases TPA-induced MMP-9 expression and invasion in MCF-7 cells. Also, brazilin suppressed NF-κB activation in TPA-treated MCF-7 cells. Taken together, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by brazilin is mediated by the suppression of the NF-κB pathway in MCF-7 cells. This result suggest brazilin provide a potential therapeutic approach for the treatment of breast cancer.

목차 (Table of Contents)

• ABSTRACT
• Introduction
• Materials and Methods
• Cells and Material
• Determination of cell viability
• Western blot analysis
• Gelatin Zymography assay
• Quantitative real-time PCR assay
• Preparation of nuclear extract
• Electrophoretic mobility shift assay (EMSA)
• Invasion assay
• Results
• Effect of brazilin on of MCF-7 cell viability
• Brazilin inhibits TPA-induced MMP-9 expression inMCF-7 cells
• Brazilin suppresses TPA-induced NF-κB DNA bindingactivity in MCF-7 cells
• Brazilin inhibits TPA-induced invasion of MCF-7cells
• Discussion
• References