정성적 중합효소연쇄반응을 이용한 가공식품 중의 유전자변형 옥수수 및 콩 함유 분석 = Qualitative detection of genetically modified maize and soy in processed foods by PCR analysis

다국어 초록 (Multilingual Abstract)

In this study, a qualitative screening method based on conventional polymerase chain reaction (PCR) assay was applied to the detection of genetically modified organisms (GMOs) in a variety of food matrices including corn starch, cereal, sweet corn, tofu, soybean milk, and soybean flour. The method was designed to amplify two different target sequences such as the 35S promoter and the NOS terminator which is present in GM-maize and -soy. All the maize and soy samples were evidenced by presence of the SSIIb and lectin gene, respectively. Out of six kinds of foodstuffs tested, the four GM-positive samples were corn starch, tofu, soybean milk, and soybean flour. Among the four GM-positive samples, only the product containing corn starch has been appropriately labelled. These results indicate that the systematic screening for a wide range of GMOs is needed to confirm that the data from PCR analysis are in accord with the mandate labelling of GMOs.