RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      참외의 PCR 최적 조건 구명 = PCR Optimization in Cucumis melo

      한글로보기

      https://www.riss.kr/link?id=A3007888

      • 0

        상세조회
      • 0

        다운로드
      서지정보 열기
      • 내보내기
      • 내책장담기
      • 공유하기
      • 오류접수

      부가정보

      다국어 초록 (Multilingual Abstract)

      This study was conducted to identify the optimized PCR condition in cucumis melo. Factorial experiments for template DNA concentration, MgCl2 concentration, amount of taq polymerase, and reaction temperature(denature, annealing, extension) were conducted. The best template DNA concentration was 40ng(0.5unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 2.5mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase)와 4.5mM(40ng template DNA and 0.5unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(60ng template DNA and 1unit taq polymerase). The best amount of taq polymerase was 0.5unit(40ng template DNA and 4.5mM MgCl2, 60ng template DNA and 2.5mM MgCl2, and 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 6 conditions(60ng template DNA, 7.0mM MgCl2, 1unit taq polymerase; 60ng template DNA, 4.5mM MgCl2, 1unit taq polymerase; 60ng template DNA, 2.5mM MgCl2, 1unit taq polymerase; 60ng template DNA, 4.5mM MgCl2, 0.5unit taq polymerase; 60ng template DNA, 2.5mM MgCl2, 0.5unit taq polymerase; and 40ng template DNA, 2.5mM MgCl2, 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 90℃, 40℃, 72℃ and 92℃, 36℃, 72℃.

      번역하기

      This study was conducted to identify the optimized PCR condition in cucumis melo. Factorial experiments for template DNA concentration, MgCl2 concentration, amount of taq polymerase, and reaction temperature(denature, annealing, extension) were conduc...

      This study was conducted to identify the optimized PCR condition in cucumis melo. Factorial experiments for template DNA concentration, MgCl2 concentration, amount of taq polymerase, and reaction temperature(denature, annealing, extension) were conducted. The best template DNA concentration was 40ng(0.5unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 2.5mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase)와 4.5mM(40ng template DNA and 0.5unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(60ng template DNA and 1unit taq polymerase). The best amount of taq polymerase was 0.5unit(40ng template DNA and 4.5mM MgCl2, 60ng template DNA and 2.5mM MgCl2, and 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 6 conditions(60ng template DNA, 7.0mM MgCl2, 1unit taq polymerase; 60ng template DNA, 4.5mM MgCl2, 1unit taq polymerase; 60ng template DNA, 2.5mM MgCl2, 1unit taq polymerase; 60ng template DNA, 4.5mM MgCl2, 0.5unit taq polymerase; 60ng template DNA, 2.5mM MgCl2, 0.5unit taq polymerase; and 40ng template DNA, 2.5mM MgCl2, 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 90℃, 40℃, 72℃ and 92℃, 36℃, 72℃.

      더보기

      목차 (Table of Contents)

      • 서언
      • 재료 및 방법
      • 1.식물재료
      • 2.Genomic DNA 분리 및 quantification
      • 3.PCR 조건
      • 서언
      • 재료 및 방법
      • 1.식물재료
      • 2.Genomic DNA 분리 및 quantification
      • 3.PCR 조건
      • 결과 및 고찰
      • 1.Template DNA 농도
      • 2.MgCl2 농도
      • 3.Taq polymerase 농도
      • 4.반응 온도
      더보기

      동일학술지(권/호) 다른 논문

      동일학술지 더보기

      더보기

      분석정보

      View

      상세정보조회

      0

      Usage

      원문다운로드

      0

      대출신청

      0

      복사신청

      0

      EDDS신청

      0

      동일 주제 내 활용도 TOP

      더보기

      주제

      연도별 연구동향

      연도별 활용동향

      연관논문

      연구자 네트워크맵

      공동연구자 (7)

      유사연구자 (20) 활용도상위20명

      이 자료와 함께 이용한 RISS 자료

      나만을 위한 추천자료

      해외이동버튼