Toluene diisocyanate(TDI) 및 Diphenylmethane diisocyanate(MDI)의 變異原性과 細胞毒性과의 相關性 硏究 = Relationship between Mutagenic Acivity and Cytotoxicity of Toluene diisocyanate and Diphenylmethane diisocyanate

다국어 초록 (Multilingual Abstract)

TDI(Toluene diisocyante) and MDI(Diphenylmethane diisocyanate) used as hardners in the production of polyurethane, showed following cytotoxicity and mutagenic activities.
1. Using TA98 without metabolic activation(S_9), TDI at the does, 78-625㎍/plate, completely inhibited TA98 growth. However TDI at the higher does than that, stimulated bacterial growth. With metabolic activation(+S_9), simillar tendency was observed but the magnitude of cytotooxicities of _S_9 was weaker than those of -S_9, Cytotoxicity pattern of MDI was similar to that of TDI, but its potency is higher than TDI's.
2. TDI and MDI appeared to be mutagenic to TA98 only after metabolic activation and this was more apparent when their cytotoxicities were diminished. Although results are not conclusive, they also showed similar tendency in the case of S_9 free condition.
3. TDI and MDI showed significant mutagenic action on TA100 only after the presence of S_9 mix, without any effect on TA100 growth.
In concusion, considering the facts that TDI and MDI have strong cytotoxicites and there was increasing tendency in the mutagenic activities accompanied decrease of cytotoxicities, it could not be ruled out that TDI and MDI are not only secondary mutagens but also primary mutagens.