The Effects of Dietary Phenolic Compounds on Cytokine and Antioxidant Production by A549 Cells

다국어 초록 (Multilingual Abstract)

Levels of inflammatory cytokines are raised in chronic obstructive pulmonary disease (COPD). A diet rich inantioxidant vitamins may protect against the development of COPD. This study examined the effects of phenolic compoundsand food sources on cytokine and antioxidant production by A549 cells. The effects of the following phenolic compounds onbasal and interleukin (IL)-1-stimulated release of IL-8, IL-6, and reduced glutathione (GSH) were examined: resveratrol; Bou-vrage, a commercially available raspberry juice (Ella Drinks Ltd., Alloa, Clacksmannanshire, UK); and quercetin 3.-sulfate.Purification of the raspberry juice by high-performance liquid chromatography gave three fractions: Fraction 1 contained phe-nolic acid and vitamin C, Fraction 2 contained flavonoids and ellagic acid, and Fraction 3 contained anthocyanins and ellag-itannins. IL-8 production was increased in the presence of IL-1 (165 vs. 6,011 pg/mL, P. .0001). None of the compoundstested had any significant effect on GSH. Resveratrol at concentrations . 50 .mol/mL significantly inhibited IL-8 and IL-6production. Similar findings were made with raspberry juice at concentrations . 25 .L/mL, and Fractions 1 and 3 were bestable to inhibit IL-8 production. Quercetin 3.-sulfate, at 25 .mol/mL, inhibited IL-8 and IL-6 production. The changes ob-served in IL-8 were paralleled by changes in tumor necrosis factor-.. Thus, phenolic compounds can significantly alter cy-tokine and antioxidant production.

참고문헌 (Reference)

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