ATPase 활성도에 미치는 카드뮴의 영향 = Effects of Cadmium on the Activity of ATPase

다국어 초록 (Multilingual Abstract)

Effects of cadmium on ATPase activities were studied in microsomal fractions obtained from liver, kidney and testis of rats.
The total ATPase activities were 5.829 ± 0.141 (S.D.) μmol/mg protein/hr, 35.992 ± 1.304μmol/mg protein/hr and 46.046 ± 0.961 μmol/mg protein/hr in microsomes of normal liver, kidney and testis, respectively. The total activities of liver and kidney microsomes were inhibited by 34.4% and 39.60%, respectively, by addition of 2 mM ouabain but that of testis was not influenced by ouabain.
The inhibition of microsomal ATPase activity by cadmium were dose-dependent in the range of concentrations from 0.05 mM to 3.0 mM. The concentrations of cadmium causing 50% inhibition (I_so) of ATPase activity were calculated by probit method. Cadmium concentrations causing 50% inhibition of total ATPase activity were found to be 0. 458 mM, 0.310 mM and 0.730 mM in liver, kidney and testis, respectively. Ouabain insensitive (Mg^++-) ATPase of liver, kidney and testis were inhibited 50% by Cd in concentrations of 1.460 mM, 0.970 mM, 0.310 mM and 0.730 mM in liver, kidney and testis, respectively. Ouabain insensitive (Mg^++- ) sitive to Cd and were inhibited 50% in microsomes of liver and kidney by Cd concentrations of 0.086 mM and 0.058 mM, respectively. Thus ouabain-sensitive (Na^+, K(^+)-) ATPasc was 17 times more susceptible to cadmium than ouabain-insensitive (Mg(^++)- ) ATPase.