Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design = Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design

다국어 초록 (Multilingual Abstract)

Plackett-Burman (PB) and Box-Behnken (BB) experimental designs were used to optimize fermentation variables for the biotransformation of glycyrrhizin (GL) to monoglucuronyl-glycyrrhetinic acid (MGGA). The PB design was first used to screen the important factors among the medium variables. The steepest ascent method was used to approach the optimum range for each of these factors. The BB design was finally used to analyze the response surfaces of the screened factors for further optimization. The optimized conditions for this system were 0.7 g/L MgSO4·7H2O, 1.19 g/L GL, and cultivation for six days. The biotransformation of GL to MGGA could reach up to 35.72%, which is a good result for this kind of transformation.

참고문헌 (Reference)

1 Clarke, K., "Therapeutic Efficacy of Anti-Lewisy Humanized 3S193 Radioimmunotherapy in a Breast Cancer Model: Enhanced Activity When Combined with Taxol Chemotherapy" 6 : 3621-3628, 2000

2 Plackett, R. L., "The Design of Optimum Multifactorial Experiments" 37 : 305-325, 1946

3 Wu, S. J., "Study on Biotransformation of Glycyrrhizin" 34 : 516-518, 2003

4 Vahid Mahdavi, "Statistical optimization for oxidation of ethyl benzene over Co-Mn/SBA-15 catalyst by Box-Behnken design" 한국화학공학회 30 (30): 2178-2185, 2013

5 Cong, J. X., "Separation of Liquiritin by Simulated Moving Bed Chromatography" 1145 : 190-194, 2007

6 Palvannan, T., "Production of Laccase from Pleurotus florida NCIM 1243using Plackett-Burman Design and Response Surface Methodology" 50 : 325-335, 2010

7 Yun, T. K., "Preventive Effect of Ginseng Intake Against Various Human Cancers: A Case-Control Study on 1987 Pairs" 4 (4): 401-408, 1995

8 Zou, S. P., "Preparative Enrichment and Separation of Glycyrrhetinic Acid Monoglucuronide from Fermentation Broths with Macroporous Resins" 47 : 1055-1062, 2012

9 Xia, S. H., "Pharmacological Action and Mechanisms of Ginkgolides B" 120 (120): 922-928, 2007

10 Jacques, P., "Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design" 77-79 : 223-233, 1999

11 Rudakewich, M., "Neurotrophic and Neuroprotective Actions of Ginsenosides Rb1and Rg1" 67 : 533-537, 2001

12 Hou, J. G., "Microbial Transformation of Ginsenoside Rg3 to Ginsenoside Rh2 by Esteya Vermicola CNU 120806" 28 : 1807-1811, 2012

13 Peng, L., "Ginsenoside Re: Pharmacological Effects on Cardiovascular System" 30 (30): 183-188, 2002

14 Liu, Y. C., "Efficient Biotransformation of Cholesterol to Androsta-1,4-diene-3,17-dione by a Newly Isolated Actinomycete Gordonia Neofelifaecis" 27 : 759-765, 2011

15 김현숙, "Effects of Ginsenosides Rg3 and Rh2 on the Proliferation of Prostate Cancer Cells" 대한약학회 27 (27): 429-435, 2004

16 Wang, Z. X., "Current status of Study on Glycyrrhizin" 22 : 796-798, 2002

17 Sirikantaramas, S., "Camptothecin: Therapeutic Potential and Biotechnology" 8 : 196-202, 2007

18 Hu, S. C., "Biotransformation of Soybean Isoflavones by a Marine Streptomyces sp. 060524 and Cytotoxicity of the Products" 25 : 115-121, 2009

19 Li, Y., "Application of Plackett-Burman Experimental Design and Doehlert Design to Evaluate Nutritional Requirements for Xylanase Production by Alter Naria Mali ND-16" 77 : 285-291, 2007

20 Yue, P. F., "Application of Plackett-Burman Design and Box-Behnken Design to Achieve Process Optimization for Geniposide Submicron Emulsion" 33 : 213-222, 2012

21 Wang, X., "Advances in Studies on Pharmacological Activities of Ginkgolides" 36 (36): 1741-1744, 2005

22 Gouiric, S. C., "7b-Dihydroxydehydroabietic acid, a New Biotransformation Product of Dehydroabietic Acid by Aspergillus Niger" 20 : 281-284, 2004