당뇨병 유발 쥐에서 신경 세포 증식에 대한 저선량 방사선의 효과 = The Effect of Low Dose Radiation on the Neuronal Cell Proliferation in Diabetic Rats

국문 초록 (Abstract)

목 적: 저선량 방사선이 당뇨쥐의 해마에서 신경 세포의 증식에 미치는 영향을 확인하기 위해서이다. 대상 및 방법: 정상 쥐를 대상으로 방사선을 조사하지 않은 군, 0.1 Gy 방사선 조사군, 10 Gy 방사선 조사군으로 나눠 방사선이 정상 신경 세포의 증식에 미치는 영향을 확인하였다. 당뇨쥐를 대상으로 한 이차 실험은 방사선을 조사하지 않은 정상 쥐, 방사선을 조사하지 않은 당뇨쥐, 0.01 Gy 방사선 조사한 당뇨쥐, 0.1 Gy를 조사한 당뇨쥐,1 Gy를 조사한 당뇨쥐, 10 Gy를 조사한 당뇨쥐로 나눠 시행하였다. 5-bromo-2’-deoxyuridine (BrdU)를 이용한 면역염색을 시행하여 쥐 해마의 치아이랑 세포의 수를 세어 비교하였다.결 과: BrdU 양성 세포의 수는 대상으로 방사선을 조사하지 않은 정상쥐, 0.1 Gy 방사선 조사한 정상쥐, 10 Gy 방사선 조사한 정상쥐에서 각각 45.96±3.42/mm2, 59.34±5.20/mm2, 19.26±2.98/mm2로 나타났다. 당뇨쥐를 대상으로한 이차 실험에서 BrdU 양성 세포의 수는 방사선을 조사하지 않은 정상 쥐, 방사선을 조사하지 않은 당뇨쥐, 0.01 Gy 방사선 조사한 당뇨쥐, 0.1 Gy를 조사한 당뇨쥐, 1 Gy를 조사한 당뇨쥐, 10 Gy를 조사한 당뇨쥐에서 각각 55.44±8.57/mm2, 33.33±6.46/mm2, 67.75±10.54/mm2, 66.63±10.05/mm2, 23.59±6.37/mm2, 14.34±7.22/mm2로 나타났다.결 론: 0.1 Gy 이하의 저선량 방사선은 당뇨쥐의 해마 치아이랑세포의 수를 증가시켜 세포 증식효과를 보이는 것을 확인하였다.

다국어 초록 (Multilingual Abstract)

Purpose: To investigate the effect of low dose radiation on neuronal cell proliferation in diabetic rats.
Materials and Methods: A group of rats (first group) were divided into three subgroups (nondiabetic control,
nondiabetic 0.1 Gy and nondiabetic 10 Gy groups) to determine the effect of radiation on normal hippocampal
neuronal cell proliferation. A further group of rats (second group) were divided into six subgroups (nondiabetic
control, diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy groups) to determine the effect of radiation on hippocampal neuronal cell proliferation under diabetic conditions. Using immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU), the number of neuronal cells in the dentate gyrus of all the groups was counted. Results: The number of BrdU-positive cells in the dentate Gyrus of the nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy subgroups of the first group were 45.96±3.42, 59.34±5.20 and 19.26±2.98/mm2, respectively. The number of BrdU-positive cells in the dentate gyrus of the diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy subgroups of the second group were 55.44±8.57, 33.33± 6.46, 67.75±10.54, 66.63±10.05, 23.59±6.37 and 14.34±7.22/mm2, respectively.
Conclusion: Low dose radiation enhances cell proliferation in the dentate gyrus of STZ-induced diabetic rats.

목차 (Table of Contents)

• 서 론
• 대상 및 방법
• 결 과
• 고안 및 결론

참고문헌 (Reference)

1 "The effect of streptozotocin-induced diabetes on cell proliferation in the rat dentate gyrus" 293 : 91-94, 2000

2 "The adaptive response in radiobiology:evolving insights and implications" 106 : 277-283, 1998

3 "Stress and hippocampal neurogenesis" 46 : 1472-1479, 1999

4 "Serotonin and hippocampal neurogenesis" 21 : 46-51, 1999

5 "Restoring production of hippocampal neurons in old age" 2 : 894-897, 1999

6 "Radiation injury and neurogenesis" 16 : 129-134, 2003

7 "Neuronal and astroglial alterations in the hippocampus of a mouse model for type 1 diabetes" 1038 : 22-31, 2005

8 "Neurogenesis in the dentate gyrus of the adult rat:age-related decrease of neuronal progenitor proliferation" 16 : 2027-2033, 1996

9 "Magnitude- and time- dependence of the effect of treadmill exercise on cell proliferation in the dentate gyrus of rats" 24 : 114-117, 2003

10 "Induction of the adaptive response by X-rays is dependent on radiation intensity" 56 : 107-118, 1989

11 "Evidence for beneficial low level radiation effects and radiation hormesis" 78 : 3-7, 2005

12 "Estrogen stimulates a transient increase in the number of new neurons in the dentate gyrus of the adult female rat" 19 : 5792-5801, 1999

13 "Effect of small dose of radiation on induction of apoptosis in murine tumors" 17 : 307-313, 1999

14 "Diabetes mellitus and risk of Alzheimer disease and decline in cognitive function" 61 : 661-666, 2004

15 "Cellular mechanisms of protection and repair induced by radiation exposure and their consequences for cell system responses" 13 : 7-20, 1995

16 "Alcohol and nicotine reduce cell proliferation and enhance apoptosis in dentategyrus" 13 : 1509-1513, 2002