질경이 추출물의 DPPH 라디칼 소거효과 및 항균활성

국문 초록 (Abstract)

질경이를 새로운 기능성 식품소재로 활용하기 위한 일환으로 차전초의 메탄올 추출물을 각종 용매를 이용하여 계통ㆍ분획한 후 DPPH 라디칼 소거효과, 환원력, 아질산염 소거효과 및 항균활성에 대하여 조사하였다. DPPH 라디칼 소거효과는 분획물 중 에틸아세테이트와 부탄올 분획물(400㎍/5 mL)에서 60% 이상 높은 라디칼 소거율과 금속에 대한 강한 환원력을 나타낸 반면, 물, 클로로포름 및 헥산 분획물은 낮은 소거효과를 나타내었다. 질경이의 각 분획물을 농도별로 첨가한 아질산염 소거효과는 에틸아세테이트와 부탄올 분획물 500 ㎍ 첨가시 70~80% 이상 높은 아질산염 소거 효과를 나타내었다. 항균활성은 에틸아세테이트, 클로로포름 및 부탄올 분획물 순으로 높게 나타났으며, 에틸아세테이트 분획물의 경우 Staphylococcus aureus, Bacillus cereus 및 Vibrio parahaemolyticus 등의 그람양성 및 음성균 모두 강한 항균효과를 나타내었다.

다국어 초록 (Multilingual Abstract)

To develop the functional food, methanol extracts of dried plantain (Plantago asiatica L.) was fractionated in hexane, chloroform, ethyl acetate, butanol and aqueous fraction. DPPH radical scavenging effect, reducing power, nitrite scavenging effect and antimicrobial activities of these fractions were investigated. Ethyl acetate fraction among these fractions showed the highest effect of all experiments. Ethyl acetate fraction showed about 60% scavenging effect on the DPPH radicals at 400 ㎍/5 mL. Nitrite scavenging effect of ethyl acetate and butanol fraction was more than 70~80% at concentration above of 500 ㎍. Among various solvent fractions from methanol extracts of plantain, ethyl acetate fraction showed the highest inhibitory effect on the microorganism such as Staphylococcus aureus, Bacillus cereus and Vibrio parahaemolyticus at 5 mg/disc.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 감사의 글
  
• 문헌

참고문헌 (Reference)

1 "한약자원식물학" 학문출판 -376, 1996

2 "신씨본초학각론" 서울 -347, 1973

3 "Studies on the active principles of wild vegetables on biotransformation of drug" 20 : 117-122, 1989

4 "Studies of products of browning reactions Antioxidative activities of products of browning reaction prepared from glucosamine" 307-315, 1986

5 "Screening of natural antimicrobial plant extract on food spoilage microorganism" 23 : 200-204, 1991

6 "Plants with liver protective activities Chemistry and pharmacology of Plantaginis Semen et Folium" 57-60, 1980

7 "Phamacological studies of Plantaginis Semen" 7 : 28-37, 1977

8 "Long-term effects of consuming foods containing psyllium seed husk on serum lipids in subjects with hypercholesterolmia." 67 : 367-376, 1998

9 "Liver-protective activities of aucubin derived from traditional oriental medicine" 102 : 189-204, 1998

10 "Korean folk medicine" Publishing center of Seoul National University Seoul -130, 1966

11 "Isolation and idenfication of antimicribial compound from plantain(Plantago asiatica L.)." 15 : 410-417, 1999

12 "Inhibition of N-nitrosamine formation in model food system" 40 : 981-985, 1975

13 "Effects of psyllium on glucose and serum lipid responses in men with type 2 diabetes and hypercholesterolmia" 71 : 1433-1438, 1999

14 "Effect of ethyl acetate fraction of plantain on experimentally- induced gastric mucosal damage and gastric ulcers in rats" 33 : 659-667, 2004

15 "Antioxidant determination by the use of a stable free radical" 25 : 1199-1120, 1958

16 "Antimicrobial effect of ethanol extracts of Quercus spp" 33 : 463-468, 2004

17 "Antimicrobial activity of some Egytion spice essential oils" 52 : 665-670, 1989

18 "Antimicrobial activity of fractional extracts form Houttuynia cordata root" 32 : 1053-1058, 2003

19 "Antimicrobial activity of clove extract by extraction solvents" 33 : 494-499, 2004

20 "Antibacterial and antioxidative activities of various extracts from Basidiomyces" 8 : 118-124, 2001

21 "A taxonomic and systematic study of genus plantago in Korea" Korea University 1996