자가 산부식 프라이머의 세포독성에 관한 실험적 연구 = In vitro cytotoxicity of self-etching primers

국문 초록 (Abstract)

자가 산부식 프라이머는 세포독성이 있는 것으로 알려져 있어 교정치료를 하는 동안 치주조직에 손상을 일으킬 수 있다. 본 연구의 목적은 자가 산부식 프라이머가 치주조직에 미치는 영향을 평가해 보고 이를 전통적인 접착법에 사용되는 프라이머와 비교하기 위하여 시행되었다. 시편은 임상에서 브라켓 접착 시 사용하는 Transbond XT Adhesive (3M Unitek, Monrovia, CA, USA)를 각각 Transbond XT Primer (3M Unitek, Monrovia, CA, USA), Clearfil SE bond (Kuraray, Osaka, Japan), Transbond Plus Self Etching Primer, Adper Prompt L-Pop (3M Unitek, Monrovia, CA, USA)과 혼합한 후 광중합하여 제작하였고, Transbond XT Adhesive를 중합한 대조군과 비교하였다. 이를 배양된 HGF-1 (Human Gingiva Fibroblast), HaCaT (Human Keratinocyte cell line), RHEK (immortalized Human Epidermal Keratinocyte)에 노출시킨 후 세포의 형태 변화를 관찰하였고, MTT assay를 시행하여 세포독성을 비교, 평가하였다. 실험결과 72시간 후 HGF-1, HaCaT, RHEK를 이용한 실험에서 모든 프라이머의 세포독성이 높게 나타나 세포 돌기의 위축, 세포 형태의 변화, 세포 수의 감소, 세포의 괴사가 관찰되었다. MTT assay실험 시 HGF-1을 이용한 실험에서 Clearfil SE Bond, Transbond XT Primer, Transbond Plus SEP, Adper Prompt L-Pop의 순으로 세포독성이 높게 나타났고, HaCaT를 이용한 실험에서 Clearfil SE Bond, Adper Prompt L-Pop, Transbond Plus SEP, Transbond XT Primer의 순으로 세포독성이 높게 나타났으며, RHEK를 이용한 실험에서 Clearfil SE Bond, Transbond XT Primer, Adper Prompt L-Pop, Transbond Plus SEP 순으로 세포독성이 높게 나타났다. 자가 산부식 프라이머는 전통적으로 사용되는 프라이머와 마찬가지로 세포독성이 유의하게 높으므로 구강내 사용시 주의가 필요하다.

다국어 초록 (Multilingual Abstract)

Objective: Several ions and components are released from self-etching primers in the oral cavity. This may cause injury to the periodontal tissues throughout orthodontic treatment. The purpose of this study was to assess the cytotoxicity of self-etching primers to HGF-1 , HaCaT, and RHEK cells. Method: Transbond XT Primer (3M Unitek, Monrovia, CA, USA), and self-etching primers, Clearfil SE Bond (Kuraray, Osaka, Japan), Transbond Plus SEP (3M Unitek, Monrovia, CA, USA), and Adper Prompt L-Pop (3M Unitek, Monrovia, CA, USA), were evaluated by MTT assay, and cellular changes were also observed, Results: In all cells after 72 hours with all primers, severe morphological changes such as atrophy and necrosis were observed. In the MTT assay using HGF-1, Clearfil SE Bond, Transbond XT Primer, Transbond Plus SEP, and Adper Prompt L-Pop were lined up in order of ascending cytotoxicity. When using HaCaT, Clearfil SE Bond, Adper Prompt L-Pop, Transbond Plus SEP, and Transbond XT Primer were lined up in order of ascending cytotoxicity. When using RHEK, Clearfil SE Bond, Transbond XT Primer, Adper Prompt L-Pop, and Transbond Plus SEP were lined up in order of ascending cytotoxicity Conclusion: The result of this study shows that care is needed because self-etching primers show cytotoxic properties similar to conventional primers.

참고문헌 (Reference)

1 "Viability measurements in mammalian cell systems" 179 : 1-7, 1989

2 "Self-etching primers-an alternative to the conventional acid etch technique?" 62 : 238-245, 2001

3 "Self-etching primer vs phosphoric acid: an alternative concept for composite-to-enamel bonding" 24 : 172-180, 1999

4 "Regional bond strengths of self-etching/self-priming adhesive systems" 26 : 609-616, 1998

5 "Reaction of fibroblasts to various dental casting alloys" 17 : 341-347, 1988

6 "Long-term cytotoxicity of orthodontic direct-bonding adhesives" 93 : 419-422, 1988

7 "Kinetic and quantitative evaluation of material cytotoxicity in vitro" 35 : 389-401, 1973

8 "In vitro cytotoxicity of orthodontic bonding resins on human oral fibroblasts" 116 : 132-138, 1999

9 "In vitro cytotoxicity of four kinds orthodontic band cements" 34 : 351-362, 2004

10 "Effect of saliva on shear bond strength of an orthodontic adhesive used with moisture-insensitive and self-etching primers" 124 : 414-419, 2003

11 "Effect of blood contamination on shear bond strength of brackets bonded with a self-etching primer combined with a resin-modified glass ionomer" 126 : 703-708, 2004

12 "Effect of a self-etch primer/adhesive on the shear bond strength of orthodontic brackets" 119 : 621-624, 2001

13 "Dental materials" Koonja Pub 67-70, 2001

14 "Cytotoxicity of modern dentin adhesives-in vitro testing on gingival fibroblasts" 63 : 53-60, 2002

15 "Cytotoxicity of Pd-Co dental casting ferromagnetic alloys" 60 : 1403-1409, 1981

16 "Cytotoxic effects of composite restorations employing self-etching primers or experimental antibacterial primers" 28 : 61-67, 2000

17 "Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity" 37 : 517-524, 1997

18 "Comparison of tetrazolium colorimetric and 51Cr release assays for cytotoxicity determination of dental biomaterials" 11 : 327-331, 1995

19 "Chemical aspects of self-etching enamel-dentin adhesives: a systematic review" 21 : 895-910, 2005

20 "Bonding of stainless steel brackets to enamel with a new self-etching primer" 122 : 274-276, 2002

21 "Bond strength comparison of 2 self-etching primers over a 3-month storage period" 126 : 709-716, 2004

22 "Biological testing of dental restorative materials in vitro-a review" 6 : 365-374, 1979

23 "Biological evaluation on glass ionomer cement" 58 : 1080-1086, 1979

24 "An evaluation and comparison of orthodontic bracket bond strengths achieved with self-etching primer" 126 : 213-219, 2004

25 "A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces" 34 : 849-853, 1955

26 "A method for the in vitro toxicity testing of dental restorative materials" 56 : 1285-1290, 1977

27 "A further testing of the effect of dental materials on growth and adhesion of animal cells in vitro" 80 : 206-212, 1972