Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E2 Generation in LPS-stimulated RAW264.7 Cells

다국어 초록 (Multilingual Abstract)

Inflammation is the basis of severe acute and chronic diseases.
This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE2) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE2 generation via suppression of iNOS and COX-2 expression.

참고문헌 (Reference)

1 Lee, T. H., "Role of inflammasome activation in development exacerbation of asthma" 4 : 187-196, 2014

2 Subash Karki, "Quantification of Major Compounds from Ixeris dentata, Ixeris dentata Var. albiflora, and Ixeris sonchifolia and Their Comparative Anti-Inflammatory Activity in Lipopolysaccharide-Stimulated RAW 264.7 Cells" 한국식품영양과학회 18 (18): 83-94, 2015

3 Ricciotti, E., "Prostaglandins inflammation. Arterioscler" 31 : 986-1000, 2011

4 Harris, S. G., "Prostaglandins as modulators of immunity" 23 : 144-150, 2002

5 Guzik, T. J., "Nitric oxide superoxide in inflammation immune regulation" 54 : 469-487, 2003

6 Iwakiri, Y., "Nitric oxide in liver diseases" 36 : 524-536, 2015

7 Selmi, C., "New treatments for inflammatory rheumatic disease" 60 : 277-288, 2014

8 Cha, M. R., "New guaiane sesquiterpene lactones from Ixeris dentata" 77 : 380-382, 2011

9 Baker, R. G., "NF-kappaB, inflammation, metabolic disease" 13 : 11-22, 2011

10 Nath, J., "Modulation of human neutrophil inflammatory responses by nitric oxide: studies in unprimed LPS-primed cells" 62 : 805-816, 1997

11 Son, D. J., "Melittin inhibits vascular smooth muscle cell proliferation through induction of apoptosis via suppression of nuclear factor-kappaB Akt activation enhancement of apoptotic protein expression" 317 : 627-634, 2006

12 Fujiwara, N., "Macrophages in inflammation" 4 : 281-286, 2005

13 Park, Eun-Kyung, "Lactic Acid Bacterial Fermentation Increases the Antiallergic Effects ofIxeris dentata" 한국미생물·생명공학회 18 (18): 308-313, 2008

14 Lee, H. Y., "Ixeris dentata-induced regulation of amylase synthesis secretion in glucose-treated human salivary glcells" 62 : 739-749, 2013

15 Yi, J. M., "Ixeris dentata green sap inhibits both compound 48/80-induced aanaphylaxis-like response IgE-mediated anaphylactic response in murine model" 25 : 5-9, 2002

16 Kim, D. S., "Ixeris dentata NAKAI reduces clinical score HIF-1 expression in experimental colitis in mice" 2013 : 671281-, 2013

17 김미리, "Ixeris dentata Extract Maintains Glutathione Concentrations in Mouse Brain Tissue Under Oxidative Stress Induced by Kainic Acid" 한국식품영양과학회 6 (6): 353-358, 2003

18 Park, S., "Isolation of two new bioactive sesquiterpene lactone glycosides from the roots of Ixeris dentata" 25 : 4562-4566, 2015

19 Ban, J. O., "Inhibition of cell growth induction of apoptosis via inactivation of NF-kappaB by a sulfurcompound isolated from garlic in human colon cancer cells" 104 : 374-383, 2007

20 Wong Zhiqin, "Inflammatory Bowel Disease-related Colorectal Cancer in the Asia-Pacific Region: Past, Present, and Future" 대한장연구학회 12 (12): 194-204, 2014

21 van Hoogmoed, L. M., "In vitro investigation of the interaction between nitric oxide cyclo-oxygenase activity in equine ventral colon smooth muscle" 34 : 510-515, 2002

22 Su, X., "Immune responses in Parkinson's disease: interplay between central peripheral immune systems" 275178-, 2014

23 Jeon, Y. D., "Effects of Ixeris dentata water extract caffeic acid on allergic inflammation in vivo in vitro" 15 : 196-, 2015

24 안은미, "Cytotoxic and ACAT-inhibitory Sesquiterpene Lactones from the Root of Ixeris dentata forma albiflora" 대한약학회 29 (29): 937-941, 2006

25 S. B. Kim, "Anti-inflammatory effects of tectroside on UVB-induced HaCaT cells" 31 : 1471-1476, 2013