RISS 검색 - 국내학술지논문 상세보기

다국어 초록 (Multilingual Abstract)

Background: Rapid detection of carbapenemase-producing Enterobacterales (CPE) isdesirable to guide antimicrobial therapy and infection control. The NG-Test Carba5 (Carba5;NG Biotech, France) rapid multiplex lateral flow immunoassay and BD MAX Check-Points CPOAssay (CPO; BD Diagnostic Systems, USA) fully automated real-time PCR assay were evaluatedfor the detection of KPC, NDM, VIM, IMP, and OXA-48-like group in a culture colony comparedto genotyping using conventional PCR.
Methods: Among the clinical isolates of carbapenem-resistant Enterobacterales(CRE) collected from 2013 to 2019, up to 20 isolates for each carbapenemase type,and approximately 60 carbapenemase-negative CRE were enrolled. Genotyping ofcarbapenemases were performed using single-target PCR for KPC, NDM, and OXA-48-likegroup and the multiplex PCR for VIM, IMP, GIM, SIM, and SPM. All isolates were tested withCarba5 and CPO. The discrepant results were resolved by single-target specific conventionalPCR or GeneXpert Carba-R Assay (Carba-R; Cepheid, USA).
Results: Of 147 CREs, 82 were CPE (55.8%) including 20 KPC, 22 NDM, 17 VIM, three IMP, and13 OXA-48-like group, and seven double carbapenemase-positive (three KPC/VIM, two NDM/VIM, one KPC/NDM, and one NDM/OXA-48-like group) isolates. Carba5 and CPO detected allCPE correctly along with two more IMP-producing CPE. The sensitivity and specificity of bothkits were equally 100% and 97%. Two false IMP-positives were confirmed IMP-positive withCarba-R and IMP-specific single-target PCR.
Conclusion: Carba5 and CPO reliably detect and differentiate five common carbapenemasesin cultured colonies. Carba5, faster and simpler, is preferred as a spot test.

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참고문헌 (Reference)

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