만성 간질환 환자의 간조직에서 B 형 간염 바이러스 항원의 발현 및 DNA 검출에 관한 연구 = Expression of HBV Antigen and Detection of HBV DNA in Liver Tissue with Chronic Liver Disease

다국어 초록 (Multilingual Abstract)

Objectives: Infection of hepatitis B virus(HBV) may develop acute or chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Several serologic markers were used in detection of HBV infection. But in order to understand the pathogenesis of chronic liver disease with HBV infection, detection of HBV marker or HBV DNA in tissue may be important. Methods: To investigate the correlation between HBV infection and chronic liver disease, immunohistochemistry for HBsAg and HBcAg, in situ DNA hybridization for HBV DNA in 141 cases of formalin fixed paraffin-embedded tissue sections of chronic liver diseases were performed. Results: The positivity of HBsAg generally increased with progression of disease. It was 60.9% in CPH, 70.8% in CAH, 75.0% in liver cirrhosis, and 50.0% in hepatocellular carcinoma. But the positivity of HBcAg decreased with progression of disease, which was 69.6% in CPH, 51.4% in CAH, 37.5% in liver cirrhosis, and 4.5% in hepatocellular carcinoma. Detection rate of HBV DNA was highly observed in chronic liver diseases, such as 91.3% in CPH, 81.9% in CAH, 70.8 % in liver cirrhosis, 95.5 % in hepatocellular carcinoma. Its positive signal was weak in tumor cells, but strong in surrounding regenerating nodule. Conclusion: These results indicate that detection of HBV DNA by in situ hybridization was more sensitive than that of HBsAg and HBcAg by immunohistochemical stain and suggest that HBU infection is closely related to the pathogenesis of chronic liver disease.