Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

다국어 초록 (Multilingual Abstract)

Bombyx mori nucleopolyhedrovirus (BmNPV) ecdysteroid
UDP-glucosyltransferase gene (egt) promoter
fragments of different lengths were amplified from
BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids
pBmegt542-luc, pBmegt309-luc and pBmegt159-
luc with luciferase (luc) driven by egt promoters were
constructed. Both in vitro and in vivo expressions
showed that BmNPV egt promoter activity requires
the transactivation of viral factor(s), and expression of
luc was detected earliest at 24 hrs post infection (pi).
BmNPV ZJ-8 homologous region 3 (hr3) increased the
expression of luc by over 1,600-fold. Molting hormone
of 1.0 ~ 2.0 mg/ml can dramatically down regulate
expression of luc. Juvenile hormone analogue of
0.5 ~ 2.0 mg/ml increased expression of luc by 145.8%
to 75.7%. Deletion assay revealed that the promoter
fragment of 159 bp contains the basal promoter structure;
Promoter fragments of 309 bp and 542 bp
showed similar but much higher transcriptional activities
than that of 159 bp, suggesting that nucleotide
from -159 to -309 nt upstream the translation initiation
site harbors the main cis-acting elements.

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