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      Mutational analysis of cucumber mosaic virus movement protein gene = 오이모자익바이러스와 상호작용하는 Plant host factor에 관한 연구

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      다국어 초록 (Multilingual Abstract)

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      The movement protein of cucumber mosaic virus (CMV) is required for cell to cell movement of viral RNA The movement of viral RNA occurs through plant intercellular connection, the plasmodesmate Viral movement protein was known to be multi-functional A series of deletion mutants of CMV movement protein gene were created to identify the functional domains The mutated movement proteins were produced as inclusion body in E coll, purified and renatured Polyclonal antibody was raised against the CMV-Kor strain (Korean isolate) movement protein expressed in E coll The ability of the truncated proteins to bind to ssRNA was aasyed by UV cross-linking and gel retardation analyses The results indicated that the domain between amino acids 118 and 160 of CMV movement protein was essential for ssRNA binding
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      The movement protein of cucumber mosaic virus (CMV) is required for cell to cell movement of viral RNA The movement of viral RNA occurs through plant intercellular connection, the plasmodesmate Viral movement protein was known to be multi-functional A...

      The movement protein of cucumber mosaic virus (CMV) is required for cell to cell movement of viral RNA The movement of viral RNA occurs through plant intercellular connection, the plasmodesmate Viral movement protein was known to be multi-functional A series of deletion mutants of CMV movement protein gene were created to identify the functional domains The mutated movement proteins were produced as inclusion body in E coll, purified and renatured Polyclonal antibody was raised against the CMV-Kor strain (Korean isolate) movement protein expressed in E coll The ability of the truncated proteins to bind to ssRNA was aasyed by UV cross-linking and gel retardation analyses The results indicated that the domain between amino acids 118 and 160 of CMV movement protein was essential for ssRNA binding

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      목차 (Table of Contents)

      • Introduction
      • Materials and Methods
      • Results and Discussion
      • Introduction
      • Materials and Methods
      • Results and Discussion
      • References
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