신규 미백제 : Selina-4(14), 7(11)-dien-8-one = New Whitening Agent : Selina-4(14), 7(11)-dien-8-one

다국어 초록 (Multilingual Abstract)

We had previously reported that Selina (selina-4(14), 7(11)-dien-8-one) was isolated from methanol extract of Afractylodes rhizome and has strong whitening activity in B16 melanoma cells. In this report, we demonstrated its action mechanism in melan-a cells, non-tumorigenic melanocytes. We also investigated the clinical efficacy of cosmetic preparation containing Selina. Selina reduced the melanin synthesis of Melan-a cells by 50% at a concentration of 10 ㎍/mL without any apparent cytotoxicity. We also found that the treatment of cells with Selina decreased tyrosinase activity by 60% at a concentration of 10 ㎍/mL but Selina was not a direct inhibitor of tyrosinase activities. To elucidate the action mechanism of Selina, we investigated the changes in mRNA and protein level of tyrosinase, TRP-1 and TRP-2 using RT-PCR and western blotting, respectively. As a result, the mRNA and protein level of tyrosinase were markedly reduced at 10 ㎍/mL of Selina without any effect on TRP-1 and TRP-2. These results suggest that Selina exerts its whitening effect mainly through regulating expression of tyrosinase. A 7 week-clinical trial using formulation containing 0.2% selina-4(14), 7(11)-dien-8-one with 20 volunteers resulted in statistically significant whitening effect (p<0.05), without any adverse effect. Based on these results, Selina (selina-4(14), 7(11)-dien-8-one) can be s useful and safe ingredient for the cleanness and brightness of skin.