Vincristine 투여가 백서 전치부 상아질 형성에 미치는 영향에 관한 주사 전자현미경적 연구 = A SCANNING ELECTRON MICROSCOPIC STUDY ON THE SHAPES OF INNER DENTINAL SURFACE IN WHITE RAT INCISORS AFTER VINCRISTINE SULFATE INJECTION

다국어 초록 (Multilingual Abstract)

It is known that vincristine sulfate, the anticariogenic drug of vinca-alkaloid family, inhibits the mitosis and injuries the cellular functions.
This study was to investigate the inner dentinal surfaces of apical portion in white rat incisors after vincristine sulfate injection. For this study, 30 Sprague-Dawley were used as the experimental animals, and divided into two experimental groups as follows: Control group. Six animals which intravenously recieved physiological salt solution (0.5mgJKg) one time. Experimental group. Twenty-four animals which intravenously recieved vincristine (0.5mg/Kg) one time.
The experimental groups were sacrificed at 3 hours, 6 hours, 12 hours, 1 day, 4 days and 7 days after the beginning of the experiment, by means of vascular perfusion with 2.5% glutaraldehyde.
The maxillary incisors including periapical tissues were removed and defatted 20% KOH solution at 60°C for 24 hours, and dehydrated with acetone. Each tooth specimen was attached on the stab for scanning electron microscopic study. Gold was coated on the each specimen in the thickness of 300A at D.C. 1400V, 6mA for 6 minutes with coating machine (Elea Ill 3). Inner dentinal surfaces of the, specimens were observed with SEM (Hitachi S-450).
The results were as follows;
1. From 3 hours group after experiment, calcospherites were decreased in number comparable
to control group, begun to be irregularly in shape and it's degree was severe with time.
2. In 1 day group after experiment, calcospherites were nearly disappeared and ability to form
dentin of odontoblasts was probably stopped.
3. In 4 days group after experiment, calcospherites were begun to be reappeared, and thereafter gradually incressed in number.
4. In 1 weeks group of after experiment, A number of calcospherites were repaired to be close to normal group, but dentinal tubules were more or less narrowed comparable to control group, and decreased in number.
5. In each experimental group, the more a number of calcospherites were abundantly increased, the more peritubular and intertubular dentin also were fine.