Candida albicans에 대한 중성 전기분해수소수의 항진균 효과 = Antifungal effects of hydrogen water on the growth of Candida albicans

다국어 초록 (Multilingual Abstract)

Objectives: This study assessed the antifungal effects of neutral electrolysis hydrogen water, an agent known for its oral anti-bacterial effects on the growth of C. albicans in vitro.
Methods: Hydrogen water, denture cleansing agent, and Listerine® were compared to a tap water control. We serially cultured C. albicans KCTC 27239 in SD media to analyze the inhibitory effect of each treatment on growth. We evaluated the data using repeated-measures ANOVA test and one-way ANOVA test, and a significant difference was defined as P<0.05.
Results: Hydrogen water significantly inhibited the growth, but not the biofilm formation, of C. albicans compared to tap water control at 1-, 5-, and 10-minute time points (P<0.05).
Conclusions: The growth of C. albicans was slower in hydrogen water than in tap water, thereby suggesting an inhibitory effect of hydrogen water. Hydrogen water may, therefore, be utilized as an antibacterial and preventive agent without any harmful effect on denture and oral tissues.

참고문헌 (Reference)

1 김영미, "알칼리 이온수의 의치 미생물에 대한 세정효과에 관한 연구" 대한치과보철학회 49 (49): 138-144, 2011

2 Calderone RA, "Virulence factors of Candida albicans" 9 : 327-335, 2001

3 Jeng ES, "The antioxidative effect of hydrogen water drinking in blood" 5 : 25-28, 2016

4 Nakagawara S, "Spectroscopic characterization and the pH dependence of bactericidal activity of the aqueous chlorine solution" 14 : 691-698, 1998

5 Baran I, "Self-reported denture hygiene habits and oral tissue conditions of complete denture wearers" 49 : 237-241, 2009

6 Kim YJ, "Pathogenic microorganisms & antibiotics" World science 349-354, 2010

7 Murdoch-Kinch CA, "Oral mucosal injury caused by denture cleanser tablets: a case report" 80 : 756-758, 1995

8 Kim J, "Inhibition of streptococcal biofilm by hydrogen water" 58 : 34-39, 2017

9 Izumi S, "Evaluation of application possibility of water containing organic acids for chemical denture cleaning for older adults" 16 : 300-306, 2016

10 Stohler C, "Etiology and occurrence of denture stomatitis: a review of the literature" 94 : 187-194, 1984

11 Waters B, "Electrolyzed oxidizing water generation methods" 7 : 1-13, 2012

12 Paranhos HF, "Effects of mechanical and chemical methods on denture biofilm accumulation" 34 : 606-612, 2007

13 Arendorf TM, "Denture stomatitis: a review" 14 : 217-227, 1987

14 Weerasekera MM, "Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development" 111 : 697-702, 2016

15 Saitoh Y, "Biological safety of neutral-pH hydrogen-enriched electrolyzed water upon mutagenicity, genotoxicity and subchronic oral toxicity" 26 : 203-216, 2010

16 Kiura H, "Bactericidal activity of electrolyzed acid water from solution containing sodium chloride at low concentration, in comparison with that at high concentration" 49 : 285-293, 2002

17 표경열, "Antifungal effect of electrolyzed hydrogen water on Candida albicans biofilm" 대한턱관절교합학회 31 (31): 212-220, 2015

18 이성훈, "Antibacterial Activity of Hydrogen-rich Water Against Oral Bacteria" 대한구강생물학회 38 (38): 81-85, 2013

19 Holladay JD, "An overview of hydrogen production technologies" 139 : 244-260, 2009

20 Budtz-Jörgensen E, "An epidemiologic study of yeasts in elderly denture wearers" 3 : 115-119, 1975

21 Shafer WC, "A textbook of oral pathology" WB Saunders Co 393-397, 1983

22 Zhang JY, "A review of hydrogen as a new medical therapy" 59 : 1026-1032, 2012