As a basic study for growth inhibition of film-forming yeasts in apple wine during storage periods, the growth conditions of Hansenula beijerinckii FY-5 which was isolated and identified in previous paper were investigated.
The optimum conditions for...
As a basic study for growth inhibition of film-forming yeasts in apple wine during storage periods, the growth conditions of Hansenula beijerinckii FY-5 which was isolated and identified in previous paper were investigated.
The optimum conditions for film-formation were pH 3.0 and temperature of 30-35℃, and sugar concentration in medium had no effect on film-formation. The thermal death of strain FY-5 was observed at 70℃ for 10 minutes. This strain was able to grow in synthetic medium contained 12% or less ethanol, but in apple wine contained 8% or less ethanol.
The strain belonged to true-film yeast, which did not required biotin for film-formation in synthetic medium, and had low tolerance for SO_(2) (70ppm). Also, the effect of amino acids and other vitamins on film-formation were investigated.