섬유소 분해균을 이용한 건조 청보리 발효사료가 돼지의 In vitro 발효 특성에 미치는 영향 = Effects of Dried Whole Crop Barley Treated with Cellulolytic Microorganisms on In Vitro Fermentation Characteristics in Swine

국문 초록 (Abstract)

본 연구는 높은 섬유소 분해력을 검증 받은 Aspergillus niger (KCCM 60357)와 Bacillus licheniformis (KCCM 40934)를 단독 및 혼합 배양한 미생물제제로 양돈용 청보리 발효사료를 제조하였을 때 사료 성상변화, in vitro 대장발효 및 전장소화율에 미치는 영향을 평가하였다. 실험 설계는 건조 청보리 (control), A. niger (control + A. niger), B. licheniformis (control + B. licheniformis) 및 Mixture (control + Aspergillus niger + Bacillus licheniformis)구로, 균주를 첨가시킨 발효사료에 따라 네 처리군으로 나누었다. 사료 성분변화는 발효사료의 일반 성분분석을 통해 확인하였으며, 그 결과 A. niger 및 B. licheniformis구에서 조단백질과 조지방 함량이 유의하게 증가하였다 (p＜0.05). In vitro 대장발효성상의 변화는 배양 후 24시간에 건물 분해율이 A. niger와 Mixture구에서 각각 55 및 57.42%로 대조구 및 B. licheniformis구와 비교하여 유의하게 높았다 (p＜0.05). 가스 발생량은 대조구에서 유의적으로 가장 높았으며, 메탄가스 농도는 모든 처리구가 대조구에 비해 증가되었다. pH는 대조구를 비롯한 모든 처리구가 6.8 로 확인되었으며, 암모니아 질소는 모든 발효사료에서 유의하게 증가되었다. Xylanase 활력은 A. niger구가 유의적으로 높았으며 모든 처리구의 활력이 증가하였다 (p＜0.05). 총 VFA 농도는 배양 후 24시간에 B. licheniformis구에서 높은 농도 (12.61mM)를 보여주며 VFA 개선효과를 나타냈다 (p＜0.05). In vitro 전장 소화율은 B. licheniformis구에서 49.61%로 대조구의 45.65% 보다 소화율이 유의적으로 증진되었다(p＜0.05). 따라서 본 실험에 사용된 미생물을 이용하여 청보리 발효사료를 제조하였을 때 전장 소화율 및 대장발효 환경 개선에 충분한 효과가 있을 것으로 기대된다.

다국어 초록 (Multilingual Abstract)

The experiment was conducted to observe the effects of dried whole crop barley treated with cellulolytic microorganisms (Aspergillus niger KCCM 60357 and Bacillus licheniformis KCCM 40934) on the chemical composition, in vitro colonic fermentation and whole tract digestibility in swine. Whole crop barley were fermented with no microorganism addition (control), A. niger, B. licheniformis and co-culture of A. niger and B. licheniformis (Mixture) for 3 days at 30℃. In the feed chemical composition, CP contents of whole crop barley treated with A. niger (7.52%) and B. licheniformis (7.77%) were significantly higher than control (6.81%) (p＜0.05). The in vitro colonic fermentation of dried whole crop barley fermented with control showed significantly higher CH₄ contents than A. niger, B. licheniformis and Mixture at 18h incubation (p＜0.05). Dry matter (DM) digestibilities of A. niger (55%) and Mixture (57.42%) treatments were significantly higher than control (43.74%) (p＜0.05). Ammonia-N was significantly increased in A. niger, B. licheniformis and Mixture relative to control at 24 hour incubation (p＜0.05). Xylanase activities in A. niger, B. licheniformis and Mixture treatments were significantly higher than control at 24 hour incubation (p＜0.05). Concentrations of total VFA were significantly increased in B. licheniformis (12.61 mM) at 24hour incubation (p＜0.05). In vitro whole tract digestibility was significantly increased in B. licheniformis (49.61%) compared with the control (45.65%) (p＜0.05). In conclusion, whole crop barley treated with cellulolytic microorganisms improved whole tract digestibility and colonic fermentation for swine.

목차 (Table of Contents)

• ABSTRACT
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• Ⅲ. 결과 및 고찰
• Ⅳ. 요약
• Ⅴ. 사사
• Ⅵ. 인용문헌

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