미성숙 복분자 과실의 항산화 효능 및 타이로시네이즈 저해 활성 평가 = Antioxidative Effect and Tyrosinase Inhibitory Activity of the Unripened Fruit Extract of Rubus coreanus Miquel

다국어 초록 (Multilingual Abstract)

In this study, the antioxidative effects and inhibitory activities of unripened fruit extract of Rubus coreanus Miquel (R. coreanus Miquel) on tyrosinase were investigated and the potential applicability as a cosmeceutical ingredients was evaluated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction of unripened fruit extract of R. coreanus Miquel. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites (FSC₅₀) of 50% ethanol extract (6.56 μg/mL) and ethyl acetate fraction (6.14 μg/mL) of unripened fruit extract of R. coreanus Miquel were higher than (+)-α-tocopherol (8.98 μg/mL), which is known as a typical hydrophobic antioxidant. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of 50% ethanol extract (0.83 μg/mL), ethyl acetate fraction (0.84 μg/mL) and aglycone fraction (1.13 μg/mL) of R. coreanus Miquel on ROS generated in Fe³⁺ -EDTA/H₂O₂ system were higher than L-ascorbic acid (1.5 μg/mL), which is known as s typical hydrophilic antioxidant. The cellular protective effect of extract and fraction of unripened fruit extract of R. coreanus Miquel on the rose bengal sensitized photohemolysis of human erythrocytes was increased in a concentration dependent manner (1 ~ 50 μg/mL). And 50% ethanol extract in 50 μg/mL showed the most protective effect among extracts (τ₅₀ = 296.3 min). The inhibitory effects on tyrosinase of ethyl acetate and agylcone fractions were higher than arbutin. These results indicate that unripened fruit extracts of R. coreanus Miquel can be applied to antioxidant scavenging ROS including radical as an alternative whitening agent to replace arbutin.

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