도라지 종자 추출물의 멜라토닌 함량 및 항산화능

국문 초록 (Abstract)

본 연구에서는 도라지 종자의 기능성을 평가하기 위해 ELISA를 이용한 멜라토닌 함량 분석, 최적 추출용매 선정, 그리고 추출물에 대한 화학적 항산화능 및 3T3-L1 세포주를 이용한 세포 내 항산화능을 조사하였다. 종자로부터 멜라토닌 추출에는 75% 에탄올이 가장 우수하였으며 이 추출물의 멜라토닌 함량은 146.8 ng/g이었다. 해당 추출물의 페놀화합물 함량은 50.2 μg GAE/g이었으며 DPPH 라디칼 소거능, ABTS 라디칼 소거능, FRAP, ORAC법으로 분석한 항산화능이 각각 519.1, 645.1, 471.1, 1,438.7 μmol TE/g으로 나타났다. 이러한 종자 추출물의 멜라토닌 및 페놀화합물함량, 그리고 항산화능은 싹이나 뿌리보다 높았다. 3T3-L1 세포주에 40 μg/mL 추출물을 처리한 세포 내 항산화능 분석에서도 종자 추출물이 21.5 unit의 항산화능을 보인 반면에 싹이나 뿌리 추출물의 항산화능은 나타나지 않았다. 이로부터 도라지 종자는 싹이나 뿌리보다 멜라토닌 함량이 높고 항산화능이 우수한 새로운 기능성 식품자원으로서의 활용가능성을 확인하였다.

다국어 초록 (Multilingual Abstract)

The melatonin content and antioxidant activity of Platycodon grandiflorum (PG) seed extract were measured and compared to those of PG sprout and root extracts. Among the solvents used to extract melatonin from seeds, 75% ethanol was better than methanol or 25, 50 or 100% ethanol (v/v), resulting in 146.8 ng melatonin per g extract by ELISA assay. The polyphenol content of seed extract prepared using 75% ethanol was 50.2 μg gallic acid equivalent (GAE)/g. This extract also exhibited dose-dependent antioxidant activity in various assays, showing 519.1 μmol Trolox equivalent (TE)/g in 2,2-diphenyl-1-picrylhydrazyl radical scavenging, 645.1 μmol TE/g in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, 471.1 μmol TE/g in ferric reducing antioxidant power, and 1,438.7 μmol TE/g in oxygen radical absorbance activity. Both the melatonin content and the antioxidant activity were highest in seed extract, followed by sprout and root extracts. Longer sprouting lowered the melatonin content and the antioxidant capacity. In a cellular antioxidant activity (CAA) test using 3T3-L1 cells, seed extract (40 μg/mL) showed 21.5 units CAA while neither sprout nor root extract exhibited any activity. These results suggest that PG seed is a novel functional foodstuff superior to its sprout and root in melatonin content and antioxidant activity.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

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