흰쥐유래의 비만세포인 RBL-2H3 세포는 다양한 Src-family kinase를 발현한다. 현재까지의 연구결과에 의하면 비만세포의 초기 활성화에 Lyn kinase가 중요한 역할을 한다고 알려져 왔다. 그러나 그 ...
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https://www.riss.kr/link?id=A103837328
이준호 (건국대학교) ; 문세환 (건국대학교) ; 고나영 (건국대학교) ; 김지완 (차의과학대학교 기초의학연구소) ; 김도균 (건국대학교) ; 김주동 (건국대학교) ; 허억 (건국대학교) ; 최완수 (건국대학교) ; Lee, Jun-Ho ; Mun, Se-Hwan ; Ko, Na-Young ; Kim, Jie-Wan ; Kim, Do-Kyun ; Kim, Joo-Dong ; Her, Erk ; Choi, Wahn-Soo
2007
English
Src-family kinase ; Lyn ; Fyn ; Fgr ; c-Src ; Mast cells
KCI등재
학술저널
350-355(6쪽)
1
0
상세조회0
다운로드국문 초록 (Abstract)
흰쥐유래의 비만세포인 RBL-2H3 세포는 다양한 Src-family kinase를 발현한다. 현재까지의 연구결과에 의하면 비만세포의 초기 활성화에 Lyn kinase가 중요한 역할을 한다고 알려져 왔다. 그러나 그 ...
흰쥐유래의 비만세포인 RBL-2H3 세포는 다양한 Src-family kinase를 발현한다. 현재까지의 연구결과에 의하면 비만세포의 초기 활성화에 Lyn kinase가 중요한 역할을 한다고 알려져 왔다. 그러나 그 세포에서 발현되는 다양한 다른 Src-family kinase의 역할은 불분명하다. 본 연구에서는 비만세포에서 다양한 Src-family kinase가 세포내 다른 곳에서 다양하게 발현되고 있다는 사실을 RT-PCR, immunoblotting 그리고 confocal microscopy 기법을 이용하여 증명하였다. 그 결과 Lyn 및 Fgr kinase는 세포막에 위치하고 c-Src 및 Yes kinase는 세포 내 과립에 존재하는 것을 알 수 있었다. 모든 Src-family kinase를 클로닝하고 과발현하여 탈과립 대한 영향을 평가하였다. 그 결과 fyn과 Fgr kinase는 비만세포에서 항원 유도의 탈과립을 증가시켰으며 반면 Lyn kinsae는 탈과립을 억제시키는 것을 확인할 수 있었다. 이러한 결과는 비만세포 초기 신호전달계에서 Fgr가 중요한 역할을 할 가능성을 제시한다.
다국어 초록 (Multilingual Abstract)
The rat RBL-2H3 mast cells contain various Src-family kinases. Previous reports with this cell line indicated that Lyn activation is an important initial signaling for the activation of the cells. However, the role and location of other Src-family kin...
The rat RBL-2H3 mast cells contain various Src-family kinases. Previous reports with this cell line indicated that Lyn activation is an important initial signaling for the activation of the cells. However, the role and location of other Src-family kinase isoforms which are expressed in the cells are not clear. In this study, we now show that isoforms of Src-family kinases, Lyn, fyn, Fgr, c-Src, and Yes are differentially expressed and located differently in the cells as indicated by RT-PCR, immunoblotting analysis, and confocal microscopy. Lyn and Fgr were located on plasma membrane but on the other hand c-Src and Yes were located on intracellular organelle. All of Src-family kinases were cloned and overexpressed for investigating the roles of the isoforms. Overexpression of Fyn and Fgr, not Lyn and c-Src, stimulated Ag-induced degranulation in the cells. Our findings strongly suggest for the first time that each of Src-family kinase isoform can regulate differentially $Fc{\varepsilon}RI$-mediated signaling in RBL-2H3 mast cells.
참고문헌 (Reference)
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1 Cissel, D. S., "Thapsigargin-induced secretion is dependent on activation of a cholera toxin-sensitive and a phosphatidylinositol-3-kinase-regulated phospholipase D in a mast cell line" 285 : 110-118, 1998
2 Kovarova, M., "Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation" 21 : 8318-8328, 2001
3 Turner, H., "Signaling through the high affinity IgE receptor FcεRI" 402 : B24-B30, 1999
4 Wedemeyer, J., "Roles of mast cells and basophils in innate and acquired immunity" 12 : 624-631, 2000
5 Kawakami, Y., "Redundant and opposing functions of two tyrosine kinases, Btk and Lyn, in mast cell activation" 165 : 1210-1219, 2000
6 Xiao, W., "Positive and negative regulation of mast cells activation by Lyn via the FcεRI" 175 : 6885-6892, 2005
7 Brown, F. D., "Phospholipase D1 localizes to secretory granules and lysosomes and is plasma-membrane translocated on cellular stimulation" 8 : 835-838, 1998
8 El Shazly, A., "Novel association of the SFKs, hck and c-fgr, with CCR3 receptor stimulation: a possible mechanism for eotaxin-induced human eosinophil chemotaxis" 264 : 163-170, 1999
9 Gresham, H. D., "Negative regulation of phagocytosis in murine macrophages by the Src kinase family member" 191 : 515-528, 2000
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16 Nishizumi, H., "Impaired tyrosine phosphorylation and Ca2+ mobilization, but not degranulation, in lyn-deficient bone marrow-derived mast cells" 158 : 2350-2355, 1997
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20 Parravinci, V., "Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation" 3 : 741-748, 2002
21 Vicentini, L., "Fgr deficiency results in defective eosinophil recruitment to the lung during allergic airway inflammation" 168 : 6446-6454, 2002
22 Gu, H., "Essential role for Gab2 in the allergic response" 412 : 186-190, 2001
23 Mocsai, A., "Adhesion-dependent degranulation of neutrophils requires the SFKs Fgr and Hck" 162 : 1120-1126, 1999
24 Way, G., "Activation of exocytosis by cross-linking of the IgE receptor is dependent on ADP-ribosylation factor 1-regulated phospholipase D in RBL-2H3 mast cells: evidence that the mechanism of activation is via regulation of phosphatidylinositol 4,5-bisphosphate synthesis" 346 : 63-70, 2000
호흡기질환 환자에서 분리한 Mycoplasma pneumoniae의 Quinolone계와 Macrolide계 항생물질에 대한 다제 저항성
식이성 폴리페놀 (-)-epigallocatechin-3-gallate가 mouse C2C12 myoblast 분화에 미치는 영향
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2011-08-03 | 학술지명변경 | 외국어명 : Korean Journal of Life Science -> Journal of Life Science | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.37 | 0.37 | 0.42 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.43 | 0.43 | 0.774 | 0.09 |