Effects of Electroacupuncture on the Alteration of Inflammation-related Proteins and Glycoconjugates in the Ankle Joint of Complete Freund’s Adjuvant-injected Rats

국문 초록 (Abstract)

목적 : 만성 염증성 질환에 대한 전침효과를 알아보기 위해 complete Freund’s adjuvant(CFA) 유발 관절염 모델의 거퇴 관절 내 염증관련 단백질 및 복합당질의 변화를 살펴보았다.
방법 : Sprague-Dawley계 흰쥐의 족부에 CFA를 주사한 다음 3일 간격으로 2㎐, 15㎐ 및 120㎐ 전침자극을 주며 부종 형성여부를 plethysmometer로 측정하여 판정하였으며 30일째 거퇴관절을 취하여 4% para- formaldehyde에 고정하고 EDTA용액에서 탈회시켜 파라핀연속 절편을 얻어 NF-κB를 비롯한 5종의 염증관련 단백질의 발현 및 복합당질 변화를 살펴보았다.
결과 : 관절연골 내 면역반응 중 연골기질은 반응이 없거나 약하고 연골세포는 NF-κBp65, I-κBα, iNOS 반응이 강하며 특히 유리연골층에서 더 현저하였으나 염증 및 전침자극에 따른 변화는 없었다. 관절낭에서 면역반응을 살펴보면 염증유발시 활액세포의 면역반응세포는 I-κBα가 감소한 반면 iNOS, IL-1β는 증가하며 특히 iNOS 증가가 현저하였으며 전침자극에 의해 iNOS가 감소하였다. 활액막조직에서 모든 면역반응이 증가하며 특히 NF-κBp65, I-κBα, iNOS 반응이 현저한데 전침자극에 의해 IL-1β를 제외한 모든 반응이 감소하였다. 복합당질 염색성은 CFA를 주사한 염증유발 흰쥐군이 정상군에 비해 감소하였다. 관절연골 중 구역간질의 중성복합당질 및 연골세포피막의 산성복합당질이 현저히 감소하였다. Lectin반응도 DBA을 제외한 모든 발현이 염증유발시 감소하였다. 그러나 전침처리에 의해 정상군과 유사한 염색성과 lectin반응을 유지하였다. 특히 구역간질의 중성복합당질과 연골세포의 sWGA와 RCA-1 반응이 현저하였다.
결론 : 만성 염증성 동물모델의 거퇴 관절 내 염증관련 단백질은 관절연골보다 관절낭에서, 복합당질의 변화는 관절연골에서 큰 변화를 보였으며 전침의 자극에 의해 이들 변화가 억제되는 것을 알 수 있다. 이상의 결과로 보아 전침처치는 염증관련 단백질 발현 및 복합당질의 변화 억제를 통해 만성 관절염 질환에 효과적임을 알 수 있다.

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