Lactobacillus plantarum CQPC02-Fermented Soybean Milk Improves Loperamide-Induced Constipation in Mice

다국어 초록 (Multilingual Abstract)

This study determined the ameliorative effects of the novel microorganism, Lactobacillus plantarum CQPC02 (LP-CQPC02), fermented in soybean milk, on loperamide-induced constipation in Kunming mice. High-performance liquid chromatography revealed that LP-CQPC02-fermented soybean milk (LP-CQPC02-FSM) had six types of soybean isoflavones, whereas Lactobacillus bulgaricus-fermented soybean milk (LB-FSM) and unfermented soybean milk (U-FSM) only had five types of soybean isoflavones. LP-CQPC02-FSM also contained more total and active soybean isoflavones than LB-FSM and U-FSM. Results from mouse experiments showed that the defecation factors (quantity, fecal weight and water content, gastrointestinal transit ability, and time to first black stool) in the LP-CQPC02-FSM-treated mice were better than those in the LB-FSM- and U-FSM-treated mice. The serum and small intestinal tissue experiments showed that soybean milk increased the motilin, gastrin, endothelin, acetylcholinesterase, substance P, vasoactive intestinal peptide, and glutathione levels and decreased the somatostatin, myeloperoxidase, nitric oxide, and malondialdehyde levels compared with the constipated mice in the control group. The LP-CQPC02-FSM also showed better effects than those of LB-FSM and U-FSM. Further results showed that LP-CQPC02-FSM upregulated cuprozinc-superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), catalase (CAT), c-Kit, stem cell factor (SCF), glial cell-derived neurotrophic factor (GDNF), neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), and aquaporin-9 (AQP9) and downregulated the expression levels of transient receptor potential cation channel subfamily V member 1 (TRPV1), inducible nitric oxide synthase (iNOS), and aquaporin-3 (AQP3) in the constipated mice. LP-CQPC02-FSM increased the Bacteroides and Akkermansia abundances and decreased the Firmicutes abundance in the feces of the constipated mice and decreased the Firmicutes/Bacteroides ratio. This study confirmed that LP-CQPC02-FSM partially reversed constipation in mice.

참고문헌 (Reference)

1 Milner P, "Vasoactive intestinal polypeptide levels in sigmoid colon in idiopathic constipation and diverticular disease" 99 : 666-675, 1990

2 Liu A, "Use of psychrotolerant lactic acid bacteria (Lactobacillus spp. and Leuconostoc spp.) isolated from Chinese traditional Paocai for the quality improvement of Paocai products" 65 : 2580-2587, 2017

3 Chien HL, "Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria" 23 : 772-778, 2006

4 Suo H, "Therapeutic effect of activated carbon-induced constipation mice with Lactobacillus fermentum Suo on treatment" 15 : 21875-21895, 2014

5 Halligan S, "The radiological investigation of constipation" 50 : 429-435, 1995

6 Furchgott RF, "The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine" 288 : 373-376, 1980

7 Hsieh MH, "The human microbiome and probiotics:Implications for pediatrics" 38 : 309-327, 2008

8 Que RY, "The Correlation of NO and NOS with functional constipation of Qi and Yin deficiency and the intervention effects of Qilang formula" 12 : 520-523, 2017

9 Gutierrez-Zepeda A, "Soy isoflavone glycitein protects against beta amyloid-induced toxicity and oxidative stress in transgenic Caenorhabditis elegans" 6 : 54-, 2005

10 Pedoto A, "Role of nitric oxide in acidosis-induced intestinal injury in anesthetized rats" 138 : 270-276, 2001

11 Chen G, "Research of Sichuan Paocai and lactic acid bacteria" 6 : 1-5, 2014

12 Nick HS, "Regulation of manganese superoxide dismutase(MnSOD)in hepatocytes and intestinal epithelial cells by inflammatory mediators" 9 : 145-, 1990

13 Akaza H, "Prostate cancer chemoprevention by soy isoflavones:Role of intestinal bacteria as the ‘‘second human genome’’" 103 : 969-975, 2012

14 Lomax AR, "Probiotics, immune function, infection and inflammation: A review of the evidence from studies conducted in humans" 15 : 1428-1518, 2009

15 Chen L, "Preventive effects of different fermentation times of Shuidouchi on diphenoxylate-induced constipation in mice" 8 : E86-, 2019

16 Qian Y, "Preventive effects of Lactobacillus plantarum YS4 on constipation induced by activated carbon in mice" 8 : 363-, 2018

17 Zhang J, "Preventive effect of Lactobacillus fermentum CQPC03 on activated carbon-induced constipation in ICR mice" 54 : E89-, 2018

18 Wang R, "Preventive effect of Dendrobium candidum Wall. ex Lindl. on activated carbon-induced constipation in mice" 9 : 563-568, 2015

19 Li G, "Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation" 13 : 1093-1100, 2017

20 Zhou JF, "Potential oxidative stress in children with chronic constipation" 11 : 368-371, 2005

21 Ambrosone CB, "Polymorphisms in genes related to oxidative stress (MPO, MnSOD, CAT) and survival after treatment for breast cancer" 65 : 1105-1111, 2005

22 Mattila JT, "Nitric oxide synthase: Non-canonical expression patterns" 5 : 478-, 2014

23 Shah V, "Nitric oxide in gastrointestinal health and disease" 126 : 903-913, 2004

24 Sun Y, "New cofactors and inhibitors for a DNA-cleaving DNAzyme: Superoxide anion and hydrogen peroxide mediated an oxidative cleavage process" 7 : 378-, 2017

25 Kon R, "Morphine-induced constipation develops with increased aquaporin-3 expression in the colon via increased serotonin secretion" 145 : 337-347, 2015

26 Ali AA, "Modulation of carbohydrate metabolism and peptide hormones by soybean isoflavones and probiotics in obesity and diabetes" 16 : 693-699, 2005

27 Tsuchihash R, "Microbial metabolism of soy isoflavones by human intestinal bacterial strains" 62 : 456-460, 2008

28 Chang TS, "Metabolism of the soy isoflavones daidzein and genistein by fungi used in the prepa-ration of various fermented soybean foods" 71 : 1330-1333, 2007

29 Deng Y, "Manipulation of intestinal dysbiosis by a bacterial mixture ameliorates loperamide-induced constipation in rats" 9 : 453-464, 2018

30 Qian Y, "Lactobacillus casei strain Shirota enhances the in vitro antiproliferative effect of geniposide in human oral squamous carcinoma HSC-3 Cells" 23 : 1069-, 2018

31 이범재, "Irritable Bowel Syndrome, Gut Microbiota and Probiotics" 대한소화기 기능성질환∙운동학회 17 (17): 252-266, 2011

32 Augustin AJ, "Intestinal, hepatic and renal production of thiobarbituric acid reactive substances and myeloperoxidase activity after temporary aortic occlusion and reperfusion" 49 : 961-968, 1991

33 Zhao Y, "Intestinal microbiota and chronic constipation" 5 : 1130-, 2016

34 Piastowska-Ciesielska AW, "Influence of a low dose of dietary soybean on bone properties and mineral status in young rats" 36 : 451-458, 2010

35 Wang XL, "Expression of aquaporin-9 in colonic mucosa of constipation-predominant irritable bowel syndrome" 24 : 1095-1097, 2015

36 Setchell KD, "Evidence for lack of absorption of soy isoflavone glycosides in humans, supporting the crucial role of intestinal metabolism for bioavailability" 76 : 447-453, 2002

37 Soudah HC, "Effect of octreotide on intestinal motility and bacterial overgrowth in scleroderma" 325 : 1461-1467, 1991

38 Liu WL, "Effect of complex food environment on production of enteriocin IN 3531 with Enterococcus faecium IN3531 as a starter in Chinese fermentation Paocai making" 884–885 : 429-432, 2014

39 Chang D, "Effect of TCM therapy on the gastrointestinal hormone and NOS in patients with functional constipation" 23 : 756-759, 2015

40 Li C, "Effect of Lactobacillus plantarum NCU116 on loperamide-induced constipation in mice" 66 : 533-538, 2015

41 Wang B, "Dietary soy isoflavones alleviate dextran sulfate sodium-induced inflammation and oxidative stress in mice" 14 : 276-282, 2017

42 Kuda T, "Dietary and lifestyle disease indices and caecal microbiota in high fat diet, dietary fibre free diet, or DSS induced IBD models in ICR mice" 35 : 605-614, 2017

43 Huang LB, "Development of functional soy bean milk for school children" 22 : 61-62, 2001

44 Xiao M, "Determination of soybean isoflavones in soybean meal and fermented soybean meal by micellar electrokinetic capillary chromatography(MECC)" 126 : 1488-1492, 2011

45 Zhou JF, "Detection of erythrocyte Cu.Zn-SOD activity in patients with functional constipation and its clinical significance" 16 : 16-18, 1994

46 Tzavella K, "Decreased substance P levels in rectal biopsies from patients with slow transit constipation" 8 : 62-63, 1996

47 Li G, "Component analysis of Pu-erh and its anti-constipation effects" 9 : 2003-2009, 2014

48 Jin SW, "Betulinic acid increases eNOS phosphorylation and NO synthesis via the calciumsignaling pathway" 64 : 785-791, 2016

49 Ikarashi N, "Aquaporins in the colon as a new therapeutic target in diarrhea and constipation" 17 : 1172-, 2016

50 Kim JE, "Anti-inflammatory response and muscarinic cholinergic regulation during the laxative effect of asparagus cochinchinensis in loperamide-induced constipation of SD rats" 20 : E946-, 2019

51 Geppetti P, "Activation and sensitisation of the vanilloid receptor: Role in gastrointestinal inflammation and function" 141 : 1313-1320, 2004