Products of the pts operon of Escherichia coli have multiple physiological roles and the operon is controlled by two promoters, P0 and P1. Expression of the pts P0 promoter that is increased during growth in the presence of glucose is also activated b...
Products of the pts operon of Escherichia coli have multiple physiological roles and the operon is controlled by two promoters, P0 and P1. Expression of the pts P0 promoter that is increased during growth in the presence of glucose is also activated by CRP-cAMP. The effects of the Mlc on the pts P0 expression were studied. In vivo transcription assay using wild type and Mlc strains grown in the presence and absence of glucose indicate that Mlc negatively regulates expression of P0, and Mlc-dependent repression is relieved by glucose in the growth medium. In vitro transcription assay using purified Mlc showed that Mlc repressed transcription from the P0 specifically.