Poster Session : PS 0374 ; Infectious Disease ; In Vitro Antiplasmodial Effect of Propolis Water Extract = Poster Session : PS 0374 ; Infectious Disease ; In Vitro Antiplasmodial Effect of Propolis Water Extract

다국어 초록 (Multilingual Abstract)

Background: Effi cacy of such antimalarial therapies (e.g ACT, eg. chloroquine, sulphadoxine- pyrimethamine, and quinine) has declined, and there is an urgent need to investigate antiplasmoidal effect of propolis as natural source novel antimalarial substance. Methods: It is a laboratory experimental study. The sample is thin blood smear infected with 3D7-strain intraerythrocytic P. falciparum in vitro. The thin blood smear made from each well in microplate tested with propolis water extract in dose of 100, 10, 1 and 0.1 μg/mL. Observations under microscope (1000-fold optical enlaregement) on sample stained with Giemsa on hour-0 and hour-48 were done, to describe the percentages of parasitemia, growth, and inhibition. Probit analysis was conducted to determine the IC50 of propolis water extract. Results: Inhibition percentages for dose of propolis water extract 100; 10, 1 and 0.1 μg/mL, are 33.33%, 13.70%, 11.76% and 5.88%, respectively. The experimental result show that propolis is related to the antiplasmodial activity. The probit analysis showed propolis IC50 is 2572,65 μg/mL. Conclusions: Propolis water extract had antiplasmodial effect on intraerythrocytic P. falciparum in vitro. Propolis has the potential to be developed as an anti-malarial drug in the future.