Cytoxan 및 Imuran 投與와 X-線 照射가 抗酸菌抗原 接種 마우스의 免疫抑制에 미치는 影響에 關한 硏究 = A Study of Immunosuppressive Effects of X-ray Irradiation, and Injection of Cytoxan and Imuran to the Mycobacterial Antigen Inoculated Mice

다국어 초록 (Multilingual Abstract)

This study was done to determine whether it is possible to inoculate with Mycobacterium, which is characterzied by prolonged life in culture and specific studies, to the mice which were immunosuppressed by X-ray irradiation and injection of Cytoxan and Imuran. The results are summerized as follows.
1. After 500r X-ray total body irradiation of the mice, the peripheral W.B.C. persisted under 4,000/㎣ for 24 days.
2. Following alternative injection of Cytoxan and Imuran(0.lmg/g of body weight) for 4days, the peripheral W. B. C. persisted under 4,000/㎣ for 9 days.
3. Following the irradiation (500r) with alternative injection of Cytoxan and Imuran (0.lmg/g body weight) for 4 days, the peripheral W.B.C. persisted under 4,000/㎣ for 28 days.
4. Following 3 days after the irradiation (500r), Mycobacterium ulcerans antigen were given to the mice. The peripheral W.B.C. increased over 4,200/㎣ 9 days after injection of the antigen.
As the above results show it was possible to produce immunosuppression in the mice for one month. So this method is acceptable in producing infection with mycobacterium which require relatively short duration of growth. But this methods is not acceptable in mycobacterium which require a long duration of growth.
Therefore it was considered recommendable another experimental method for producing infection in mice with Mycobacterium lepra.