균체외단백질 생산균 Bacillus brevis P176-2 균주의 동정과 배양조건 = Identification and Cultural Conditions of Extracellular Cellular Protein Producing Bacterium Bacillus brevis P176-2

다국어 초록 (Multilingual Abstract)

Identification and influence of the cultural conditions upon extracellular protein production by Bacillus brevis P176-2 isolated from soil were investigated.
The morphological, physiological and cultural characteristics of strain P176-2 were studied according to the methods of Bergey's manual. Based on the results obtained in thses experiments, strain P176-2 was identified as Bacillus brevis. The extracellular protein ws efficiently produced when added to 1.0% peptone as nitrogen source. Optimum initial pH and temperature for extracellular protein production were 7.5 and 40℃ respectively. Higher concentration of magnesium ion stimulated growth, but prevented extracellular protein production. Optimum concentration of potassium phosphate was found to be 0.2%. This strain produced 5.38mg/ml of extracellular protein when cultured for 48 hours under optimum conditions. The extracellular protein produced under optimum conditions contained relatively large amounts of aspartic acid, lysine and arginine, but cystine and methionine were very low.

목차 (Table of Contents)

• Ⅰ. 서 론
• Ⅱ. 실험재료 및 방법
• 1. 사용균주
• 2. 배양방법
• 3. 증식도 측정
• 4. 균체외단백질 정량
• 5. 균주의 동정
• 6. 아미노산조성 분석
• Ⅲ. 실험결과 및 고찰
• 1. 균체외단백질생산 균주의 동정
• 2. 질소원의 영향
• 3. 배양온도와 초기 pH의 영향
• 4. 무기염류의 영향
• 5. 배양시간에 따른 변화
• 6. 균체외단백질의 아미노산 조성
• Ⅳ. 결 론
• 참고문헌
• Summary