녹용의 약효 성분에 관한 연구 ( Ⅴ ) 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여 = Biochemical Studies on Antler ( Cervus nippon taiouanus ) ( Ⅴ ) A Study on Glycolipids and Phospholipids of Antler Velvet Layer and Pantocrin

다국어 초록 (Multilingual Abstract)

The lipid soluble fraction of Antler velvet layer (cervus nippon taiouanous) was extracted and compared to that of pantocrin (ethanol preparation of Antler, commercially available). Lipid soluble components (801㎎/24.5g from Antler velvet ayer and 979.1㎎/143㎖ from pantocrin) were fractionated by gel filteration using a sephadex CT-25 into neutral lipids, sulfatides, cerebrosides, phospholipids and non-lipid components (lipoprotein and peptide). A silicic acid and a DEAE-sephadex A-25 column chromatography were followed from refractionation and purification. Each fraction was identified by thin layer chromatography using standard materials. Typical lipid soluble fraction of Antler contained 65.3% neutral lipid, 5.2% glycolipid, 12.9% phospholipid, 6.1% ganglioside and 2.9% non-lipid components. The glycolipid was composed of 63.5% cerebroside and 36.5% sulfatide. The cerebroside fraction of the Antler velvet layer showed four spots while pantocrin gave only one spot on a thin layer chromatogram. Among the four spots of Antler cerebroside two had almost same migration with standard but two had quite different mobility compared to the standard. The latter fractions were hydrolyzed by 0.025M methanolic HCl in order to desulfate. The two unknown materials were identified as sulfatides by the method of IR spectroscopy and thin layer chromatography. By two dimensional thin layer chroma tography, it was found that phospholipid of thr. Antler velvet layer were composed of phosphatidylethanolamine, sphingomyelin, phosphatidylcholine, lysophospahtidylethanolamine, and lysophosphatidylcholine and phosphatidic acid. On the other hand pantocrin contained phosphatidylcholine and sphingomyelin as phospholipids.