간장 허혈 및 재관류시 vitamin C와 E가 간장기능에 미치는 영향 = Effect of Vitamin C and E on Hepatic Biliary and Microsomal Function in Hepatic Ischemia/Reperfusion

다국어 초록 (Multilingual Abstract)

Abstract - This study was done to investigate the effect of vitamin C and E on hepatic biliary and microsomal function during ischemia and reperfusion. Rats were treated with vitamin C, E or with vehicle(saline) and then subjected to 60 min no-flow hepatic ischemia in vivo. Control animals were time-matched sham ischemic animals. After 1 or 5 hr of reperfusion, bile was collected, blood was obtained from the abdominal aorta, and liver microsomes were isolated. In vehicle-treated ischemic rats, serum ALT levels peaked at 5 hr and were significantly attenuated by vitamin C, E individually and combination of vitamin C and E treatment. Similarly hepatic wet weight-to-dry weight ratio was increased in the vehicle-treated ischemic group. Vitamin C and E combination minimized the increase in this ratio. Lipid peroxidation was elevated in vehicle-treated ischemic group, but this elevation was also inhibited by vitamin C or E, respectively. Bile flow and cholate output, but not bilirubin output, were markedly decreased by ischemia/reperfusion. Vitamin C treatment restored the secretion. Cytochrome P-450 content was decreased by ischemia/reperfusion and restored by vitamin E treatment to the level of sham operated group. Aminopyrine N-demethylase activity was decreased and aniline p-hydroxylase activity was increased by ischemia/reperfusion. These changes were prevented by vitamin C or E treatment, but not by vitamin C and E combination treatment. Our findings suggest that vitamin C or E individually significantly ameliorates these ischemia/reperfusion-induced hepatic damages and there is no evidence of synergism between vitamin C and E in our system.