더덕 화분의 형태학적 특성에 관한 연구 = A Study on the Morphological Characteristics of Codonopsis lanceolata pollen

국문 초록 (Abstract)

본 연구에서는 더덕 화기발달 초기단계에서 완전 성숙단계까지 화분의 외부 형태학적 특성을 조사, 분석하고, 칼륨-특이적형광 염료인 PBFI (Potassium-benzofuran isophthlate)와 형광 현미경을 사용하여 더덕의 화분발달 과정 중 칼륨의 분포 양상을 조사하여 화분발달과 칼륨의 관계를 분석하였다.

다국어 초록 (Multilingual Abstract)

This study was conducted to get basic information on the morphological characteristics of Codonopsis lanceolata (Sieb. & Zucc.) Trautv. pollen. Morphological characteristics were observed using scanning electron microscopy. Distribution of potassium on the pollen surface was investigated using the potassium sensitive fluorescent dye, potassium-binding benzofuran isophthalate (PBFI) and confocal laser microscopy. C. lanceolata pollen is a single medium-sized grain. The pollen viewed at the equatorial axis is oblate or suboblate spheroidal shape. There are very short spinules over the surface forming a particular surface. It has 9 colpates distributed evenly on the surface.
Colpus length covers more than 2/3 of the distance from pole to pole. The edges of each aperture are lined with spinules. The apertures were visible on the immature pollen from the young closed flower. On the mature pollen, the apertures were fully developed and prominent forming a complete 9-colpate shape. Fluorescence of PBFI was observed evenly on the pollen surface except the aperture areas in both immature and mature pollens, indicating that potassium is distributed evenly on the pollen surface except the aperture areas. This finding is different from the previous observations of heavy distribution of potassium on the aperture areas in barley and sesame pollens.

목차 (Table of Contents)

• Ⅰ. 서언 = 1
• Ⅱ. 연구사 = 3
• Ⅲ. 재료 및 방법 = 11
• 1. 공시 재료 = 11
• 2. 실험 방법 = 12
• Ⅳ. 결과 및 고찰 = 13
• 1. 화분의 형태학적 특성 = 13
• 2. 화분에서의 칼륨-특이적 형광의 분포 = 17
• Ⅴ. 적요 = 20
• 인용 문헌 = 21