Recombinant DNA and Protein Vaccines for Foot-and-mouth Disease Induce Humoral and Cellular Immune Responses in Mice

다국어 초록 (Multilingual Abstract)

Foot-and-mouth disease virus (FMDV) is a small single-
stranded RNA virus which belongs to the family Picornaviridae,
genus Apthovirus. It is a principal cause of FMD
which is highly contagious in livestock. In a wild type virus infection,
infected animals usually elicit antibodies against
structural and non-structural protein of FMDV. A structural
protein, VP1, is involved in neutralization of virus particle,
and has both B and T cell epitopes. A RNA-dependent RNA
polymerase, 3D, is highly conserved among other serotypes
and strongly immunogenic, therefore, we selected VP1 and
3D as vaccine targets. VP1 and 3D genes were codon-optimized
to enhance protein expression level and cloned into
mammalian expression vector. To produce recombinant protein,
VP1 and 3D genes were also cloned into pET vector.
The VP1 and 3D DNA or proteins were co-immunized into 5
weeks old BALB/C mice. Antigen-specific serum antibody
(Ab) responses were detected by Ab ELISA. Cellular immune
response against VP1 and 3D was confirmed by ELISpot
assay. The results showed that all DNA- and protein-immunized
groups induced cellular immune responses, suggesting
that both DNA and recombinant protein vaccine administration
efficiently induced Ag-specific humoral and cellular
immune responses.

다국어 초록 (Multilingual Abstract)

Foot-and-mouth disease virus (FMDV) is a small single-
stranded RNA virus which belongs to the family Picornaviridae,
genus Apthovirus. It is a principal cause of FMD
which is highly contagious in livestock. In a wild type virus infection,
infected animals usually elicit antibodies against
structural and non-structural protein of FMDV. A structural
protein, VP1, is involved in neutralization of virus particle,
and has both B and T cell epitopes. A RNA-dependent RNA
polymerase, 3D, is highly conserved among other serotypes
and strongly immunogenic, therefore, we selected VP1 and
3D as vaccine targets. VP1 and 3D genes were codon-optimized
to enhance protein expression level and cloned into
mammalian expression vector. To produce recombinant protein,
VP1 and 3D genes were also cloned into pET vector.
The VP1 and 3D DNA or proteins were co-immunized into 5
weeks old BALB/C mice. Antigen-specific serum antibody
(Ab) responses were detected by Ab ELISA. Cellular immune
response against VP1 and 3D was confirmed by ELISpot
assay. The results showed that all DNA- and protein-immunized
groups induced cellular immune responses, suggesting
that both DNA and recombinant protein vaccine administration
efficiently induced Ag-specific humoral and cellular
immune responses.

참고문헌 (Reference)

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