출생후 성숙에 따라 나타나는 흰쥐 십이지장 및 공장 점막의 alkaline phosphatase 활성에 대한 연구 = Alkaline phosphatase activity of the Duodenal and Jejunal Mucosa in the Postnatal Albino Rats

다국어 초록 (Multilingual Abstract)

The alkaline phosphatase, which is the hydrolytic enzyme of the phosphate salt in alkaline solution, acts on differentiation and maturation of cells and tissues, absorption and metabolism of nucleic acid and proteins. The author has investigated the change in the activity of alkaline phosphatase of the duodenal and jejunal mucosa of the rats according to biochemical and physiological maturation during early postnatal life. The experimental animals, which were obtained from litters deliveried, normally and randomly sampled adults, were sacrificed at the 1st, 5th, 10th, 20th, and 30th day after birth.
The specimens of duodenum and jejunum were fixed in 10%-neutral formalin at 4℃ and sectioned 10㎛ thickness in cryostat. The activity of the alkaline phosphatase was evaluated histochemically by Gomori's method and the incubation time of sliced specimens was 30 minutes.
The results were as follows.
1. In the neonatal rats of the 1st day after birth, the activities of the alkaline phosphatase were moderately positive in the epithelium and lamina propria of villi and weakly positive in the intestinal glands and its surrounding lamina propria of the duodenal mucosa. ANd the activities of alkaline phosphatase in the mucosa of the jejunum were moderately positive in the epithelium of the villi and weakly positive in the intestinal glands and lamina propria.
2. In the neonatal rats of the 5th day after birth, the activities of the alkaline phosphatase were moderately positive in the epithelium of apical and middle portion of villi. traceablely positive in the epithelium of basal portion and lamina propria of the mucosa of the duodenum. And the activities of alkaline phosphatase were moderately positive in the epithelium of apical and basal portion of the villi and weakly positive in the epithelium of middle portion of the villi. the intestinal glands and lamina propria of the jejumum.
3. In the neonatal rats of the 10th day after birth, the activities of alkline phosphatase were moderately or strong positive in the epithelium of the villi and weakly positive in the epithelium of the villi and weakly positive in the intestinal gland and lamina propria of jejunal mucosa.
4. In the rats of the 20th day after birth, the activities of alkaline phosphatase were strong or moderately positive in the epithelium of the villi, moderately positive in lamina propria of the core of the villi and the weakly positive in the intestinal glands and its surrunding lamina propria of duodenum. And the activities of alkaline phosphatase in the mucosa of the jejunum were strong positive in the epithelium of basal portion of the villi, intestinal gland and lamina propria of the villi and weakly positive in the lamina propria surrounding intestinal glands.
5. In the rats of the 30th day after birth, the activities of alkaline phosphatase were moderately or strong positive in the epithelium of the villi, modereate positive in the lamina propria of the villi and weakly positive in the intestinal glands and its surrounding lamina propria of the duodenal mucosa. And the activity of alkaline phosphatase was strong positive in the epithelium of apical portion of villi of the mucosa of the jejunum. Activity was decreased gradually and in the epithelium of basal portion of the villi activity was weakly positive. The activities were moderately positive in the intestinal glands and weakly positive in surrounding lamina propria of the glands and weakly positive in surrounding lamina propria of the glands of the jejunal mucosa.
6. In the rats of the adulthood, the activities of the alkaline phosphatase were strong positive in the epithelium of the villi and weakly positive in the intestinal glands and lamina propria of the duodenum. And the activities of alkaline phosphatase were moderately positive in the epithelium of the villi and weakly positive in the intestinal glands and lamina propria of the mucosa of the jejunum.
Consequently, it is suggested that the activities of the alkaline phosphatase in the mucosa of duodenum and jejunum were increased by differentiation, growth, maturation and the changes of diet during early postnatal life, of the rats.