옥수수 유묘기 한발 스트레스 평가를 위한 발현마커 후보군 탐색 = Assessment of the Candidate Genes of Expression Markers Associated with Drought Stress in Maize Seedlings

다국어 초록 (Multilingual Abstract)

Drought stress during the seedling stage has a disastrous effect on the growth of maize. The purpose of this study was to assess the developed expression markers that are related to drought stress in maize. For the selection of expressed genes by drought stress, co-expression analysis was carried out using published microarray data of drought stress in maize (Zea mays L.) seedlings. Six consensus modules were based on 4,770 stress responsive genes differentially expressed in drought stress, and the royal blue module was chosen. Thirty genes were selected according to different expression patterns between susceptible and tolerant types. Drought stress treatments were performed on both Ki3 and Ki11. Ki3 and Ki11 are widely known drought-susceptible and -tolerant types, respectively. At first, the 30 selected genes were compared to Ki3 and Ki11 using qRT-PCR. The gene expression values of eight genes (BU050895, BF728598, CK827168, CO524848, AF457983, CF037152, AJ606944, and BG836522) were significantly tolerant types rather than susceptible types in the roots. After applying the eight above-mentioned genes to nine cultivars, a different pattern was detected between susceptible and tolerant types. The results of the present study will show the possibility of developing novel expression markers and the application for various maize varieties.

목차 (Table of Contents)

• Abstract
• 서언
• 재료 및 방법
• 결과 및 고찰
• 적요
• REFERENCES

참고문헌 (Reference)

1 Tollenaar M, "Yield potential, yield stability and stress tolerance in maize" 75 : 161-169, 2002

2 Blanc G, "Widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes" 16 : 1667-1678, 2004

3 De La Cruz M, "Where do seedlings go? A spatio-temporal analysis of seedling mortality in a semi-arid gypsophyte" 31 : 720-730, 2008

4 Langfelder P, "WGCNA: an R package for weighted correlation network analysis" 9 : 2008

5 SanMiguel P, "The paleontology of intergene retrotransposons of maize" 20 : 43-45, 1998

6 Buckler ES, "The genetic architecture of maize flowering time" 325 : 714-718, 2009

7 Paterson AH, "The Sorghum bicolor genome and the diversification of grasses" 457 : 551-556, 2009

8 Schnable PS, "The B73 maize genome: complexity, diversity, and dynamics" 326 : 1112-1115, 2009

9 Zhao Y, "Systematic Analysis of Sequences and Expression Patterns of Drought-Responsive Members of the HD-Zip Gene Family in Maize" 6 : e28488-, 2011

10 Leach KA, "Primary root elongation rate and abscisic acid levels of maize in response to water stress" 51 : 157-172, 2011

11 Arumuganathan K, "Nuclear DNA content of some important plant species" 9 : 208-218, 1991

12 Hao ZF, "Meta-analysis of constitutive and adaptive QTL for drought tolerance in maize" 174 : 165-177, 2010

13 Meeks M, "Measuring maize seedling drought response in search of tolerant germplasm" 3 : 135-147, 2013

14 Wang L, "Large-scale screening for Aegilops tauschii tolerant genotypes to phosphorus deficiency at seedling stage" 204 : 571-586, 2015

15 Zhang W, "Identification of maize long non-coding RNAs responsive to drought stress" 9 : 2014

16 Fan CY, "Genome-wide identification and functional analysis of lincRNAs acting as miRNA targets or decoys in maize" 16 : 2015

17 Thirunavukkarasu N, "Genome-wide expression of transcriptomes and their co-expression pattern in subtropical maize (Zea mays L.) under waterlogging stress" 8 : 2013

18 Liu SX, "Genome-wide analysis of ZmDREB genes and their association with natural variation in drought tolerance at seedling stage of Zea mays L" 9 : 2013

19 Yu J, "Genetic design and statistical power of nested association mapping in maize" 178 : 539-551, 2008

20 Chardon F, "Genetic architecture of flowering time in maize as inferred from quantitative trait loci meta-analysis and synteny conservation with the rice genome" 168 : 2169-2185, 2004

21 Betran FJ, "Genetic analysis of inbred and hybrid grain yield under stress and nonstress environments in tropical maize" 43 : 807-817, 2003

22 Manoli A, "Evaluation of candidate reference genes for qPCR in maize" 169 : 807-815, 2012

23 Thornsberry JM, "Dwarf8 polymorphisms associate with variation in flowering time" 28 : 286-289, 2001

24 Lehtimaki N, "Drought stress-induced upregulation of components involved in ferredoxin-dependent cyclic electron transfer" 167 : 1018-1022, 2010

25 Brugiere N, "Cytokinin Oxidase Gene Expression in Maize Is Localized to the Vasculature, and Is Induced by Cytokinins, Abscisic Acid, and Abiotic Stress" 132 : 1228-1240, 2003

26 Gulli M, "Comparison of drought stress response and gene expression between a GM maize variety and a near-isogenic non-GM variety" 10 : 2015

27 Gale MD, "Comparative genetics in the grasses" 95 : 1971-1974, 1998

28 Swigonova Z, "Close split of sorghum and maize genome progenitors" 14 : 1916-1923, 2004

29 Mulo P, "Chloroplast-targeted ferredoxin-NADP+ oxidoreductase (FNR): Structure, function and location" 1807 : 927-934, 2011

30 Banziger M, "Breeding for drought and nitrogen stress tolerance in maize: from theory to practice" 2000

31 Myles S, "Association mapping: critical considerations shift from genotyping to experimental design" 21 : 2194-2202, 2009

32 Krill AM, "Association and linkage analysis of aluminum tolerance genes in maize" 5 : 2010

33 Paterson AH, "Ancient polyploidization predating divergence of the cereals, and its consequences for comparative genomics" 101 : 9903-9908, 2004

34 Unterseer S, "A powerful tool for genome analysis in maize: development and evaluation of the high density 600 k SNP genotyping array" 15 : 2014