A5E promotes Cell growth Arrest and Apoptosis in Non Small Cell Lung Cancer

다국어 초록 (Multilingual Abstract)

A5E is complex of several medicinal herb ethanolextracts. The aim of this study is investigating the anticancer effectfor non-small cell lung cancer. The antitumor effects of A5E onNCI-H460 were examined by regulation of cell proliferation,apoptosis, cell cycle arrest, mitochondrial membrane potential(Δψm), and apoptosis-related protein. Cell proliferation was measuredby MTS assay. Apoptosis induced by A5E was confirmed byAnnexin V-fluorescein isothiocyanate (FITC)/Propidium Iodide(PI) staining, and cell cycle arrest was measured by PI staining.
NF-κB translocation was detected by immunofluorescence andMMP (Δψm) was measured by JC-1 staining. The expression ofextrinsic pathway molecules such as FasL and FADD wereelevated, and procaspase-8 was processed by A5E. In addition,intrinsic pathway related molecules were altered. The Bcl-2 andBcl-xl levels decreased, Bax increased, and cytochrome C wasreleased. In addition, the mitochondrial membrane potentialcollapsed, and caspase-3 and poly-(ADP-ribose) polymerase wereprocessed by A5E. Moreover, A5E affected the cellular survivalpathway involving phosphatidylinositol 3-kinase (PI3K)/Akt andNF-κB. PI3K and Akt were downregulated, also NF-κBexpression was decreased, and nuclear translocalization wasinhibited by A5E. These results suggested that A5E delaysproliferation, inhibit cell cycle progression and induce apoptosis inhuman lung cancer cell. We conclude that A5E is a potentialanticancer agent for human lung carcinoma.

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