마우스 림프구증식과 GM-CSF생성에 미치는 Berberine의 효과 = Effects of Berberine on Lymphocyte Proliferation and GM-CSF Production in Mice.

다국어 초록 (Multilingual Abstract)

Berberine, an alkaloid initially isolated from chinese herbal medicine, has antibiotic activities against a variety of organisms including bacteria, viruses, fungi, protozoans, and chlamydia. Furthermore, berberine has shown a number of beneficial effects, including anti-tumor, anti-inflammation, and vasodilatory effects. In this work we have investigated the effects of berberine on lymphocyte proliferation and GM-CSF production in mice. Mouse splenocytes were incubated with berberine and concanavalin A(Con A) to observe the effects on cell proliferation. The culture supernatants of splenocytes exposed to berberine, berberine plus LPS, and berberine plus Con A were harvested to assay GM-CSF. The cell proliferation of nice splenocytes exposed to berberine only($1{\mu}g/ml$) was increased significantly more than PBS(control) group. But the Con A-induced cell growth was inhibited by berberine. The GM-CSF production from mice splenocyte culture exposed to berberine only was increased in comparison with PBS(control) group, but the production of it with LPS or Con A was inhibited by berbeline. The present findings may explain lympocyte proliferating and regulating effects of berberine.

참고문헌 (Reference)

1 Costello, R. T., "Therapeutic use of Granulocyte-macrophage colony-stimulating factor (GM-CSF). A review of recent experience" 32 : 403-408, 1993

2 Marinova, E. K., "Suppression of experimental autoimmune tubulointerstitial nephritis in BALB/c mice by berberine" 48 : 9-16, 2000

3 Colmbo, M. L, "Pharmacological activities of Chelidonium majus L" 33 : 127-134, 1996

4 Hano, K., "Pharmacogical studies on metabolism of cancer tissues: Pharmacological studies on carcinostatic effects of some plant components and their derivatives (1)" 48 : 443-450, 1957

5 Sun, D., "Influence of berberine sulfate on synthesis and expression of Pap fimbrial adhesion in uropathogenic Escherichia coli" 32 : 1274-1277, 1988

6 Lopez, A. F., "Human interleukin-3 inhibits the binding of granulocyte- macrophage colony-stimulating factor and interleukin-5 to basophils and strongly enhances their functional activity" 145 : 69-77, 1990

7 Dedhar, S., "Human granulocyte-macrophage colony-stimulating factor is a growth factor active on a variety of cell types of nonhemopoietic origin" 85 : 9253-9257, 1988

8 Gasson, J. C., "Human granulocyte-macrophage colony-stimulating factor (GM- CSF): regulation of expression" 338 : 27-41, 1990

9 Bussolino, F., "Granulocyte-macrophage colony-stimulating factors induce human endothelial cells to migrate and proliferate" 337 : 471-473, 1989

10 Ruef, C., "Granulocyte-macrophage colony-stimulating factor: pleiotropic cytokine with potential clinical usefulness" 12 : 41-62, 1990

11 Cebon, J. S., "Granulocyte-macrophage colony-stimulating factor for cancer treatment" 51 : 177-188, 1994

12 Markowicz, S., "Granulocyte- macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro" 85 : 955-961, 1990

13 Chi, C. W., "Flowcytometric analysis of effect of glucocorticoid receptors in human Hepatoma HepG2 cells" 54 : 2099-2107, 1994

14 Chang, K. S. S., "Berberine- induced morphological differentiation and down-regulation of c-Ki-ras protooncogene expression in human teratocarcinoma cells" 55 : 103-108, 1990

15 Amin, A. H., "Berberine sulfate: antimicrobial activity, bioassay, and mode of action" 15 : 1067-1076, 1969

16 Sun, D., "Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane" 32 : 1370-1374, 1988

17 Kuo, C. L., "Berberine complexes with DNA in the berberine-induced apoptosis in human leukemic HL-60 cells" 93 : 193-200, 1995

18 Iizuka, N., "Anticachectic effects of Coptidis rhizoma, an antiinflammatory herb, on esophageal cancer cells that produce interleukin 6" 158 : 35-41, 2000

19 Ghosh, A. K., "Amastigote inhibition and mode of action of berberine" 60 : 404-413, 1985