The experiments were conducted to identify several factors influencing mesophyll protoplast isolation, culture and plant regeneration of rape(Brassica napus cv. ‘Hanrayuchae’) The results obtained were summarized as follows ; It was required to p...
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https://www.riss.kr/link?id=A105116944
1986
-
520
KCI등재후보
학술저널
42-49(8쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
The experiments were conducted to identify several factors influencing mesophyll protoplast isolation, culture and plant regeneration of rape(Brassica napus cv. ‘Hanrayuchae’) The results obtained were summarized as follows ; It was required to p...
The experiments were conducted to identify several factors influencing mesophyll protoplast isolation, culture and plant regeneration of rape(Brassica napus cv. ‘Hanrayuchae’) The results obtained were summarized as follows ;
It was required to preplasmolyse the mesophyll tissue before enzyme treatment to increase yield and stability of protoplasts.
The most healthy protoplasts could be obtained when the tissue was treated with enzymes containing macerozyme 1.0% and cellulase 2.0% for 4 hours.
Sustained cell division was obtained after culturing the protoplasts in liquid 8P-KM medium supplemented with 2.4-D 0.2mg/ℓ, NAA 1.0mg/ℓ, BAP 0.5mg/ℓ, glucose 0.4M, and mannitol 0.1M.
First cell division was observed 5 days after plating and was sustained to eventually produce cell colonies after another 16 days. When micro-calli 4-5 weeks after plating were transferred to B₅ basal medium with NAA 1.0mg/ℓ, BAP 0.5mg/ℓ, sucrose 30g/ℓ and agarose 6g/ℓ, green calli were proliferated to be at least 0.5-1.0cm in diameter 8 weeks after initial plating.
Multiple shoots were induced on MS medium supplemented with BAP 1.0mg/ℓ and NAA 0.01mg/ℓ. After transferring differentiated shoots onto MS medium without hormones, intact plants were eventually produced.
수도 (水稻) 품종간 교잡에 있어서 간장의 (稈長) 유전분리 10
재식양식, 종자친 대 화분친 비율 및 밀도가 단옥수수 단교잡종 채종량에 미치는 영향