女性生殖器組織의 植物凝集素 結合에 關한 免疫酵素組織化學的 硏究 = A Study on Lectin Histochemistry in Female Genital Organs

다국어 초록 (Multilingual Abstract)

Recently, receptor specific lectins are being widely and usefully applief in the study of cell surface topography.
And binding of lectin by cell surface receptors can be demonstrated at three levels of sensitivity: by agglutination, immunohistology or electron microscopy and radioactive markers.
Especially, immunoenzyme histology using horseradish peroxidase as a conjugate has emerged as a powerful diagnositic tool with advatage of applicability to fixed tissue sections.
With the materal obtained under controlled conditions, the specificity and sensitivity of immunoperoxidase staining have been reported to be comparable or superior to those of immunofluorescence.
However, reports on lectin immunoenzyme histology are fragmentary applying only to a single organ or tissue in a certain species.
Therefore, the author collected formalin fixed, paraffin embedded female genital organes of human, rabbit and mouse and applied 8 kinds of lectins showing different carbohydrate specificity to compare differences in lectin reactivity between interspecies of interorgan.
To demonstrate lectin reactivity, samples were stained by Avidin-Biotin-Complex method using biotinylated lectins obtained form Vector stain Lab. Inc.
The results obtained are as following:
1. Endometrial surface eqithelium revealed specific binding sites for RCA I & PTA in mouse, UEA I in rabbit and Con-A in human and mouse.
2. Endometrial glandular eqithelium showed specific binding sites for UEA I in rabbit, RCA I in mouse, and Con-A and PTA both in human and mouse.
3. Epithelium of the oviduct revealed specific binding sites for UEA I in rabbit, SBA in inouse, and Con-A both in rabbit and mouse.
4. Luteal cells of the ovary exhibited specific binding sites for DBA in rabbit, WGA in human and mouse, and RCA I in human and rabbit.
5. Granulosa cell showed specific binding sites only for Con-A in mouse.
6. Theca cell revealed specific binding sites for WGA and PNA in human, PTA in human and rabbit.
7. Zona pellucida disclosed specific binding sltes for RCA I in rabbit, WGA and DBA in mouse.
As for as normal cells of female reproductive organs of humn, rabbit and mouse are concerned, above findings suggest that the pattern of carbohydrate consitituent of the cell membrane are substantially different between interspecies and interorgan.