Detection, Characterization and Antibiotic Susceptibility of Clostridioides (Clostridium) difficile in Meat Products

다국어 초록 (Multilingual Abstract)

Clostridioides (Clostridium) difficile is a Gram (+), anaerobic, spore forming, rod shaped bacterium that can produce toxin. The objective of this study is to reveal the presence of C. difficile in meat products, to analyze the ribotype diversity by PCR and to evaluate the antibiotic susceptibility of isolated strains. The organism was isolated in 22 out of 319 (6.9%) examined meat product samples and 9 out of 22 (40.9%) isolates were identified as RT027 and all isolates had the ability of toxin production. In terms of antibiotic susceptibility, all isolates were susceptive to amoxicillin-clavulanic acid, tetracycline and vancomycin and 21 (95.4%) isolates to metronidazole. On the other hand, imipenem and cefotaxim resistance was observed in all. In conclusion, the results of this comprehensive study conducted in Turkey deduced the presence of C. difficile in different meat products. Therefore, these products can be evaluated as a potential contamination source of C. difficile from animals to humans especially for elders, youngsters, long terms wide spectrum antibiotic used and immuno-suppressed individuals.

목차 (Table of Contents)

• Introduction Materials and Methods Results and Discussion Conclusion Conflicts of Interest Acknowledgements Author Contributions Ethics Approval References

참고문헌 (Reference)

1 Drudy D, "Toxin A-negative, toxin B-positive Clostridium difficile" 11 : 5-10, 2007

2 Troiano T, "Toxigenic Clostridium difficile PCR ribotypes in edible marine bivalve molluscs in Italy" 208 : 30-34, 2015

3 Hampikyan H, "The prevalence of Clostridium difficile in cattle and sheep carcasses and the antibiotic susceptibility of isolates" 139 : 120-124, 2018

4 Deng K, "Survival of Clostridium difficile spores at low temperatures" 46 : 218-221, 2015

5 Stubbs S, "Production of actin-specific ADP-ribosyltransferase(binary toxin)by strains of Clostridium difficile" 186 : 307-312, 2000

6 Curry SR, "Prevalence of Clostridium difficile in uncooked ground meat products from Pittsburgh, Pennsylvania" 78 : 4183-4186, 2012

7 Şeyma Şeniz Ersöz, "Prevalence of Clostridium difficile Isolated from Beef and Chicken Meat Products in Turkey" 한국축산식품학회 38 (38): 759-767, 2018

8 Romano V, "Prevalence and genotypic characterization of Clostridium difficile from ruminants in Switzerland" 59 : 545-548, 2012

9 De Boer E, "Prevalance of Clostridium difficile in retailed meat in the Netherlands" 144 : 561-564, 2011

10 Rodriguez C, "Presence of Clostridium difficile in pigs and cattle intestinal contents and carcass contamination at the slaughterhouse in Belgium" 166 : 256-262, 2013

11 Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing"

12 Pasquale V, "Occurrence of toxigenic Clostridium difficile in edible bivalve molluscs" 31 : 309-312, 2012

13 Esfandiari Z, "Occurrence of Clostridium difficile in seasoned hamburgers and seven processing plants in Iran" 14 : 283-, 2014

14 Lemee L, "Multiplex PCR targeting tpi(triose phosphate isomerase), tcdA(Toxin A), and tcdB(Toxin B)genes for toxigenic culture of Clostridium difficile" 42 : 5710-5714, 2004

15 Rodriguez C, "Multilocus sequence typing analysis and antibiotic resistance of Clostridium difficile strains isolated from retail meat and humans in Belgium" 42 : 166-171, 2014

16 Von Abercron SMM, "Low occurrence of Clostridium difficile in retail ground meat in Sweden" 72 : 1732-1734, 2009

17 Susick EK, "Longitudinal study comparing the dynamics of Clostridium difficile in conventional and antimicrobial free pigs at farm and slaughter" 157 : 172-178, 2012

18 Bidet P, "Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing" 175 : 261-266, 1999

19 Weese JS, "Detection and characterization of Clostridium difficile in retail chicken" 50 : 362-365, 2010

20 Rodriguez C, "Clostridium difficile in young farm animals and slaughter animals in Belgium" 18 : 621-625, 2012

21 Metcalf DS, "Clostridium difficile in vegetables, Canada" 51 : 600-602, 2010

22 Metcalf D, "Clostridium difficile in seafood and fish" 17 : 85-86, 2011

23 Songer JG, "Clostridium difficile in retail meat products USA" 15 : 819-821, 2009

24 Harvey RB, "Clostridium difficile in retail meat and processing plants in Texas" 23 : 807-811, 2011

25 Rahimi E, "Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran" 5 : 128-131, 2015

26 Jobstl M, "Clostridium difficile in raw products of animal origin" 138 : 172-175, 2010

27 Bouttier S, "Clostridium difficile in ground meat, France" 16 : 733-735, 2010

28 Simango C, "Clostridium difficile in broiler chickens sold at market places in Zimbabwe and their antimicrobial susceptibility" 124 : 268-270, 2008

29 Pires RN, "Clostridium difficile contamination in retail meat products in Brazil" 22 : 345-346, 2018

30 European Committee for Antimicrobial Susceptibility Testing, "Clinical breakpoint - bacteria (v 9.0)"

31 Pelaez T, "Characterization of swine isolates of Clostridium difficile in Spain: A potential source of epidemic multidrug resistant strains?" 22 : 45-49, 2013

32 Indra A, "Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping" 57 : 1377-1382, 2008

33 Varshney JB, "Characterization of Clostridium difficile isolates from human fecal samples and retail meat from Pennsylvania" 11 : 822-829, 2014

34 Libby DB, "Bacteremia due to Clostridium difficile: Review of the literature" 13 : e305-e309, 2009

35 Thitaram SN, "Antimicrobial susceptibility of Clostridium difficile isolated from food animals on farms" 227 : 1-5, 2016

36 Candel-Perez C, "A review of Clostridioides [Clostridium] difficile occurrence through the food chain" 77 : 118-129, 2019