Capsaicin is isolated in a pure state from the aceton extract of 1~5g. of powdered Capsaicin, by paper partition Chromatography using benzine saturated with methanol as the developing agent. The blue colorating was formed by 3% Phosphomolybdic acid an...
Capsaicin is isolated in a pure state from the aceton extract of 1~5g. of powdered Capsaicin, by paper partition Chromatography using benzine saturated with methanol as the developing agent. The blue colorating was formed by 3% Phosphomolybdic acid and 0.1N Sodium hydroxide and its extinction coefficient(E) was measured after 1 hour by the Beckman Model DV spectrophotometer at 730mμ(in 1cm cell), Quantitative determination of capsaicin was attempted by calculating the content from the standard Concentration-extinction coefficient regression equation, r=1241 E=40.02(cf. Table), for Capsaisin,
Vanillin, used by North as the standard for the determination of capsium, Was also submitted to the some quantitative determination, and it was found usable in place of Capsaicin.
The relationship between capsaicin and vanillin is as follows : Capsaicin content
(r)=Vanillin content(r)/2.300