High-level Expression of Thermus filiformis DNA Polymerase-encoding Gene = Thermus filiformis DNA polymerase 遺傳子의 高發現

다국어 초록 (Multilingual Abstract)

This study covers the development of expression vector and host system for the high-level expression of Thermus filiformis (Tfi) DNA polymerase gene. The PCR amplified fragments of the Tfi DNA polymerase gene were cloned into expression vectors, pJR and pET-22b(+). The transformed cells with recombinant plasmids were sonicated and heat-treated, and then partially purified enzymes were assayed. The expression level in the E. coli BL21(DE3) / pET-22b(+) system reached 116.5 units/㎖, roughly a 2.24-fold increase compared with production in the E. coli MV1184 / pJR system. The example of high-level expression of Tfi DNA polymerase in this study contributes to mass production and the increasement of industrial values of DNA polymerases that have become a powerful enzyme in molecular biology and biotechnology.