Rapid Quantification of Cellular Flavonoid Levels using Quercetin and a Fluorescent Diphenylboric Acid 2-amino Ethyl Ester Probe

다국어 초록 (Multilingual Abstract)

Flavonoids are abundant in fruits and vegetablesand have various biological activities. A rapid, sensitivemethod for assessing cellular quercetin levels is necessarybecause cellular levels are often low. A new method formeasuring cellular flavonoid levels is proposed usingdiphenylboric acid 2-aminoethyl ester (DPBA), a fluorescentprobe, and quercetin, a representative flavonoid. Thisspectrofluorometry-based assay is simple and allows rapidquantification of the cellular quercetin content with highsensitivity. CHO-K1 cells were incubated with quercetinfor 5 min, and then the cellular quercetin concentration wasassayed using DPBA spectrofluorometry. Results, comparedwith high-performance liquid chromatography (HPLC),thin layer chromatography (TLC), and conventionalspectrophotometry measurements, were highly correlatedwith HPLC data (R>0.99). The method is more sensitivethan TLC and conventional spectrophotometry. Spectrofluorometricmeasurement using DPBA is a simple, rapid,and sensitive method for assessing cellular quercetin levels.

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