Inhibitory Effects of Silsosangami on the Platelet Aggregation

다국어 초록 (Multilingual Abstract)

The thrombosis importantly came to the front as the risk factor of these circulation system's disease. SilsoSanGami(SSG) was used for investigating the inhibitory effect on platelet-activating factor-induced platelet aggregation about drugs that used to improvement various symptoms created by the thrombosis in oriental medicine. In this study, the water-extracted SSG was investigated for its possible antithrombotic action on platelets. The antithrombotic activity of water-extracted SSG was deduced from its ability to suppress platelet aggregation, ATP-exocytosis, and the generation of prostaglandin E_2 and thromboxane A_2 by human platelets, stimulated with arachidonic acid. Water-extracted SSG dose-dependently suppressed the aggregation of human platelets, the release of endogenous ATP, and the formation of PGE_2 and TXB_2, both the latter usually detected to estimate the activity of COX and TXS, respectively. Since the IC_50 values necessary to inhibit COX (115 ㎍/㎖ SSG) and TXS(74 ㎍/㎖ SSG) were in the same range, inhibition of COX is suggested to be the primary target of water-extracted SSG, thus suppressing the formation of PGE_2 which is metabolized by TXS to TXA_2. We considerated that SSG has practical applicational value of clinical trial in the thrombosis caused by platelet aggregation.