자궁경부 편평상피암에서 in vitro , ex vivo 및 in vivo 수소 핵 자기공명분광상의 특성 분석 = Analysis of in vitro, ex vivo and in vivo H Magnetic Resonance Spectroscopy Pattern in Squamous Cell Carcinoma of Uterine Cervix

다국어 초록 (Multilingual Abstract)

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다국어 초록 (Multilingual Abstract)

Objective: To evaluate the potential value of in vitro, ex vivo, and localized in vivo 'H Magnetic resonance spectroscopy(MRS) for detecting and characterizing squamous cell carcinoma of uterine cervix. Materials and Methods: Eleven cases of squamous cell carcinoma and 6 cases of normal cervical tissue underwent in vitro and ex vivo 'H MRS. In vitro analysis was carried out after processing the specimen with dual phase extraction method. In ex vivo test, at least 500 p 1 of tissue specimen was directly analyzed by 100.14 MHz NMR spectrometer (Bruker 11.7T AMX, Germany) without any processing procedure. In addition, 29 patients with squamous cell carcinoma of uterine cervix and 6 normal women were examined by in vivo 'H MRS. Localized in vivo 'H MR spectra of the human uterine cervix were obtained using PRESS sequence with homebuilt endovaginal surface coil. Surface coil T2 weighted images were used as the localizer images where voxel (1-3 mm3) was located to contain the cancer area only. The following spectral acquisition parameters were used: SW=2500 Hz, SI=2048, TR=3.0 sec, TE=20/135 msec, NEX=2, and NS=36. HO signal suppressed down to 96-99% by 3-pulse CHESS technique. The spectra were processed with 0.5 Hz of exponential line broadening, zerofilling of 8K followed by linear phasing. Results: The intense resonance signal at 0.9 ppm and 1.3 ppm by lipid component of triglyceride was visible in all cases (11/11) of squamous cell cancer of cervix both in vitro and ex vivo test. But in 6 normal cervical tissues, such peaks were not detected at all. The in vivo spectrum of the cervical cancer was comparable with that in in vitro and ex vivo spectrum. In the in vivo spectra of the cervical cancer, the resonance peaks at 0.9 ppm, 1.3 ppm, 3.0 ppm, and 3.2 ppm from CH3 and CH2 groups of neutral mobile lipid (triglyceride), creatine, and choline containing residues respectively, were consistent with results reported earlier. The resonance peak at 1.3 ppm which is from acyl chains of triglyceride was characteristically intense and seen in 25 of 29 cancer patients. But triglyceride peak at 1.3 ppm was not detected in normal cervix (sensitivity 86.2%; specificity 100%; positive predictive value 100%; negative predictive value 60%). Conclusion: The study shows that H MRS can effectively discriminate squamous cell cancer from normal cervix in most cases. It also demonstrates the feasibility of localized in vivo 'H MRS technique as a new diagnostic modality in the detection of squamous carcinoma of uterine cervix.