광물성 섬유의 수산이온기 생성이 흰쥐 폐포대식세포의 세포독성과 적혈구 세포막의 지질과산화에 미치는 영향 = The Effect of Hydroxyl Radical Generation on the Cytotoxicity of Alveolar Macrophage and Lipid Peroxidation of erythrocyte Membrane by Mineral fibers

다국어 초록 (Multilingual Abstract)

There is some evidence suggesting that the mechanism of pulmonary toxicity of natural fibrous silicate, asbestos, is related to the generation of oxygen-based free radical. Especially the hydroxyl radical(·OH) is believed to be the most reactive one. ·OH is a potent toxic oxidant, ·OH is which means highly cytotoxic, genotoxic and carcinogenic. Nevertheless there has been no previous report about ·OH generation from man-made mineral fibers except asbestos.
In this paper we tested the hypothesis that both cytotoxicity of alveolar macrophage and lipid peroxidation of erythrocytes are associated with ·OH production by mineral fibers in vitro.
The results were as follows:
1. ·OH production in vitro was progressively increased by the concentration dependent pattern with the same mineral fiber. The production of ·OH in vitro by man-made mineral fiber was markedly decreased compared with that of natural mineral fiber except rock wool.
2. Lipid peroxidation of mineral fiber-exposed erythrocyte suspension was significantly increased compared with that of phosphate-buffered saline(PBS) control group. Lipid peroxidation in amosite and chrysotile group was larger than that of other mineral fibers.
3. There was a significantly positive correlation between ·OH production in vitro and lipid peroxidation of mineral fiber-exposed erythrocyte.
4. All of mineral fibers were related to the cytotoxicity of alveolar macrophage in concentration-dependent fashion. Chrysotile and amosite were more cytotoxic than crocidolite. Among man-made mineral fibers, ceramic fiber was the most cytotoxic to alveolar macrophage. The viability of alveolar macrophage exposed to asbestos was markedly decreased compared with that of man-made mineral fiver at the same concentration.
5. There was the significant negative correlation between the production of .OH and the viability of alveolar macrophages.
From these results, we can conclude that the production of ·OH in vitro was strongly correlated with the cytotoxicity of alveolar macrophage and the lipid peroxidation in mineral fiber-exposed erythrocyte. And we documented that man-made mineral fiber could generate .OH in vitro less than asbestos.