한국산 청개구리 ( Hyla japonica ) Mitochondrial DNA 의 집단간 및 집단내 유전적 변이 = The Intra - and Interpopulational Genetic Variation in Mitochondrial DNA of Korean Hyla japonica

다국어 초록 (Multilingual Abstract)

Mitochondrial DNAs (mtDNAs) of Korean Hyla japonica (11 populations) and Japanese H. japonica (1 population) were analysed to investigate inter- and intrapopulation of genetic variation. Purified mtDNA of Korean H. japonica were digested with each of 11 restriction enzymes and 8 enzymes for Japanese H. japonica mtDNA. The average genome size of mtDNA was 20.30±0.3 kb in Korean species, while 20.07±0.8kb in Japanese one. For the analysis, level of fragment homology (F) and nucleotide sequence divergence (p) were estimated from comparisons of digestion profiles. Among 11 populations of Korean species, the average F and p values were 0.722±0.15 (0.246-0.970) and 0.020±0.01 (0.002-0.084) while between Korean populations and Japanese one they revealed 0.172±0.04 (0.114-0.225) and 0.109±0.01 (0.082-0.133), respectively. The nucleotide sequence divergence between 11 populations of Korean species was 2% which indicated low genic variation among populations. However, the value between Korean and Japanese populations revealed more higher. 11.5% which indicated that comparison of H. japonica from two countries showed higher genic variations.